# hmmscan :: search sequence(s) against a profile database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query sequence file: AA/23726_FusoPortal_Gene_1055.faa # target HMM database: Pfam-A.hmm # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: 23726_FusoPortal_Gene_1055 [L=223] Description: # 1164136 # 1164804 # -1 # ID=1_1055;partial=00;start_type=ATG;rbs_motif=AGGAGG;rbs_spacer=5-10bp;gc_cont=0.277 Scores for complete sequence (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Model Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8.3e-16 57.4 0.0 2.9e-12 46.0 0.0 2.2 2 Cupin_2 Cupin domain 7.4e-06 25.8 0.0 0.00024 20.9 0.0 2.1 2 AraC_binding AraC-like ligand binding domain 0.0026 17.8 0.0 2.8 8.0 0.0 2.3 2 Ectoine_synth Ectoine synthase 0.0038 16.8 0.0 0.018 14.6 0.0 1.9 2 Cupin_3 Protein of unknown function (DUF861) 0.0076 16.3 0.0 0.33 11.1 0.0 2.3 2 CENP-C_C Mif2/CENP-C like ------ inclusion threshold ------ 0.03 13.8 0.0 0.12 11.8 0.0 2.0 2 Cupin_1 Cupin Domain annotation for each model (and alignments): >> Cupin_2 Cupin domain # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 9.5 0.0 0.00026 0.72 19 57 .. 55 92 .. 37 104 .. 0.79 2 ! 46.0 0.0 1.1e-15 2.9e-12 2 65 .. 154 216 .. 153 221 .. 0.91 Alignments for each domain: == domain 1 score: 9.5 bits; conditional E-value: 0.00026 EEEEEEEEESEEEEEECTEEEEEETTEEEEE-TT-EEEE CS Cupin_2 19 edeffyVleGegeltvdgeeveleaGdsvyfpagtpHrf 57 + ++y+ G ge+ +++++ +++ Gd++ + a+ + 23726_FusoPortal_Gene_1055 55 N-RYYYCFNGSGEVSIENNKKHISNGDFLEVLAHNNYSI 92 5.789************************9988766655 PP == domain 2 score: 46.0 bits; conditional E-value: 1.1e-15 EEEEETTEEEEEECSSSEEEEEEEEESEEEEEECTEEEEEETTEEEEE-TT-EEEEEE-SSS-E CS Cupin_2 2 lvelppGesapshrHpgedeffyVleGegeltvdgeeveleaGdsvyfpagtpHrfrNtgdepa 65 ++++ +Ge+ +h pg +++ Vl+Geg+++vdg+++++++G+s + pa+ pH+++ ++ + 23726_FusoPortal_Gene_1055 154 IMSFWKGETLDPHKAPGD-ALVTVLDGEGKYIVDGKTFIVKKGESTVLPANIPHAVEAVENFKM 216 688999***********8.*************************************99886655 PP >> AraC_binding AraC-like ligand binding domain # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 2.8 0.0 0.035 96 28 65 .. 59 96 .. 58 127 .. 0.86 2 ! 20.9 0.0 8.5e-08 0.00024 17 65 .. 164 212 .. 154 219 .. 0.89 Alignments for each domain: == domain 1 score: 2.8 bits; conditional E-value: 0.035 EEEEE-EEEEETTEEEEE-TT-EEEE-TTS-EEEEE-T CS AraC_binding 28 yvteGtgrlrlngrtydlgpGdlfLlppgepHdyesds 65 y+ G+g ++++++ +++ Gd + + + ++ s++ 23726_FusoPortal_Gene_1055 59 YCFNGSGEVSIENNKKHISNGDFLEVLAHNNYSIKSSD 96 8889*******************999999999998887 PP == domain 2 score: 20.9 bits; conditional E-value: 8.5e-08 EEETT-SEEEEEEEEE-EEEEETTEEEEE-TT-EEEE-TTS-EEEEE-T CS AraC_binding 17 pphvhdeyvihyvteGtgrlrlngrtydlgpGdlfLlppgepHdyesds 65 +ph + ++ v++G+g++ ++g+t+ +++G+ lp + pH e+ + 23726_FusoPortal_Gene_1055 164 DPHKAPGDALVTVLDGEGKYIVDGKTFIVKKGESTVLPANIPHAVEAVE 212 688888889999********************************99987 PP >> Ectoine_synth Ectoine synthase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 7.7 0.0 0.0012 3.5 59 104 .. 58 101 .. 15 109 .. 0.84 2 ! 8.0 0.0 0.001 2.8 59 104 .. 174 217 .. 160 221 .. 0.88 Alignments for each domain: == domain 1 score: 7.7 bits; conditional E-value: 0.0012 EEEEE--EEEEETTT--EEEE-TTEEEEETTT-EEEEEESS-EEEE CS Ectoine_synth 59 vycieGegeveeledgevyeikpgtlYlldkhdkHllrakkelrlv 104 yc++G+gev + ++ +i++g++ + h+++ +++++ l+l+ 23726_FusoPortal_Gene_1055 58 YYCFNGSGEVSIE-NNKK-HISNGDFLEVLAHNNYSIKSSDTLKLI 101 69********875.4444.599999999999999999999999887 PP == domain 2 score: 8.0 bits; conditional E-value: 0.001 EEEEE--EEEEETTT--EEEE-TTEEEEETTT-EEEEEESS-EEEE CS Ectoine_synth 59 vycieGegeveeledgevyeikpgtlYlldkhdkHllrakkelrlv 104 v +++Geg+ dg+++ +k+g+ +l + H ++a ++++++ 23726_FusoPortal_Gene_1055 174 VTVLDGEGKYI--VDGKTFIVKKGESTVLPANIPHAVEAVENFKMM 217 56788888754..58**************************99987 PP >> Cupin_3 Protein of unknown function (DUF861) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -1.0 0.0 0.5 1.4e+03 33 54 .. 64 82 .. 54 86 .. 0.64 2 ! 14.6 0.0 6.4e-06 0.018 26 67 .. 171 211 .. 163 216 .. 0.84 Alignments for each domain: == domain 1 score: -1.0 bits; conditional E-value: 0.5 ECEEEEEETTTEEEEEETTEEE CS Cupin_3 33 eGevkvtpeggetvelraGDlv 54 +Gev+++ ++ +++ GD++ 23726_FusoPortal_Gene_1055 64 SGEVSIEN---NKKHISNGDFL 82 45555554...67778888875 PP == domain 2 score: 14.6 bits; conditional E-value: 6.4e-06 EEEEEEEECEEEEEETTTEEEEEETTEEEEE-TTEEECCCEE CS Cupin_3 26 dEfcyileGevkvtpeggetvelraGDlvvlpkglsgtwdvs 67 d ++++l Ge k+ + +g+t+ +++G + vlp++ + ++ 23726_FusoPortal_Gene_1055 171 DALVTVLDGEGKYIV-DGKTFIVKKGESTVLPANIPHAVEAV 211 66899**********.*****************986665554 PP >> CENP-C_C Mif2/CENP-C like # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 11.1 0.0 0.00012 0.33 37 73 .. 58 94 .. 42 98 .. 0.91 2 ? 2.8 0.0 0.045 1.3e+02 39 66 .. 176 203 .. 164 217 .. 0.82 Alignments for each domain: == domain 1 score: 11.1 bits; conditional E-value: 0.00012 EEEEESEEEEEETTEEEEEETT-EEEE-TT-EEEEEE CS CENP-C_C 37 FyVikGrvevtvsetsFvitkgsvfqvPrgneysieN 73 +y ++G ev++++++ i++g+ v + n+ysi+ 23726_FusoPortal_Gene_1055 58 YYCFNGSGEVSIENNKKHISNGDFLEVLAHNNYSIKS 94 78999*****************************985 PP == domain 2 score: 2.8 bits; conditional E-value: 0.045 EEESEEEEEETTEEEEEETT-EEEE-TT CS CENP-C_C 39 VikGrvevtvsetsFvitkgsvfqvPrg 66 V+ G+ + v + F++ kg + P+ 23726_FusoPortal_Gene_1055 176 VLDGEGKYIVDGKTFIVKKGESTVLPAN 203 7788888889999999999999999986 PP >> Cupin_1 Cupin # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -0.5 0.0 0.28 7.7e+02 59 66 .. 59 66 .. 18 130 .. 0.62 2 ? 11.8 0.0 4.5e-05 0.12 54 103 .. 170 212 .. 140 222 .. 0.71 Alignments for each domain: == domain 1 score: -0.5 bits; conditional E-value: 0.28 EEEESEEE CS Cupin_1 59 yvlqGegr 66 y+ +G+g 23726_FusoPortal_Gene_1055 59 YCFNGSGE 66 55555555 PP == domain 2 score: 11.8 bits; conditional E-value: 4.5e-05 S-EEEEEEESEEEEEEEETTSS.EEEEEEEETTEEEEE-TT-EEEEEEES CS Cupin_1 54 ateiayvlqGegrvgfvvpgngnrdfhkklreGdvfvvPaglvhfqynsg 103 ++ vl+Geg +v + + +++G+ v+Pa+++h++ + + 23726_FusoPortal_Gene_1055 170 GDALVTVLDGEGK-YIVDGK------TFIVKKGESTVLPANIPHAVEAVE 212 5566677777777.444444......355*****************9995 PP Internal pipeline statistics summary: ------------------------------------- Query sequence(s): 1 (223 residues searched) Target model(s): 16712 (2907032 nodes) Passed MSV filter: 518 (0.0309957); expected 334.2 (0.02) Passed bias filter: 349 (0.0208832); expected 334.2 (0.02) Passed Vit filter: 41 (0.00245333); expected 16.7 (0.001) Passed Fwd filter: 6 (0.000359023); expected 0.2 (1e-05) Initial search space (Z): 16712 [actual number of targets] Domain search space (domZ): 6 [number of targets reported over threshold] # CPU time: 0.27u 0.26s 00:00:00.53 Elapsed: 00:00:00.34 # Mc/sec: 1906.67 // [ok]