# hmmscan :: search sequence(s) against a profile database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query sequence file: AA/23726_FusoPortal_Gene_1257.faa # target HMM database: Pfam-A.hmm # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: 23726_FusoPortal_Gene_1257 [L=264] Description: # 1375902 # 1376693 # -1 # ID=1_1257;partial=00;start_type=ATG;rbs_motif=GGAG/GAGG;rbs_spacer=5-10bp;gc_cont=0.226 Scores for complete sequence (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Model Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 5e-10 39.1 19.0 1.3e-09 37.7 1.2 2.4 3 NicO High-affinity nickel-transport protein ------ inclusion threshold ------ 0.014 15.2 0.3 0.39 10.5 0.0 2.2 2 DUF2182 Predicted metal-binding integral membrane protein ( 0.074 12.9 0.1 0.074 12.9 0.1 3.4 3 DUF1980 Domain of unknown function (DUF1980) 4.3 6.4 11.4 14 4.6 11.4 2.0 1 Zip ZIP Zinc transporter Domain annotation for each model (and alignments): >> NicO High-affinity nickel-transport protein # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -4.3 0.4 2 8.4e+03 59 59 .. 17 17 .. 5 34 .. 0.47 2 ! 37.7 1.2 3.1e-13 1.3e-09 4 107 .. 57 161 .. 54 169 .. 0.89 3 ! 13.3 4.0 8.6e-06 0.036 180 275 .. 167 255 .. 160 261 .. 0.72 Alignments for each domain: == domain 1 score: -4.3 bits; conditional E-value: 2 NicO 59 v 59 + 23726_FusoPortal_Gene_1257 17 I 17 1 PP == domain 2 score: 37.7 bits; conditional E-value: 3.1e-13 NicO 4 liglsflyGvrHAvdagHgkaiirklmqagktalkrGillslgsslvvvlvavllvvaavlvlr.ltslsetgaaigteiasF 85 + flyG+ H +++gHgk+ + ++ +++k + + +l s++ + ++++ a +lv ++ +++ +++ ++ t +++ 23726_FusoPortal_Gene_1257 57 MLFFTFLYGIVHSFGPGHGKTLVLTYSVKEKLNFPKLLLVSFLIAYLQGVSAYILVKFIINLSDkASMMLFYDLDNRTRLIAS 139 56678***********************************************9999999999888999999999999999999 PP NicO 86 llvilllnllllrklrklfrkl 107 +l+il++ ++ lr+ + 23726_FusoPortal_Gene_1257 140 ILIILIGLYNIYSVLRNKCCEH 161 9999999999998888766555 PP == domain 3 score: 13.3 bits; conditional E-value: 8.6e-06 NicO 180 silalpalfavGlrlcdgadivlmfaalnwaflggrklvyamsitgtsitvalliglivtlklvakkfalsgslwaaiislel 262 + +l + + +Gl++c+g+++vl+f ++ ++ + +++ms g++ ++ ++ +l+ t+k + ++ + + + l 23726_FusoPortal_Gene_1257 167 VKNILGFSIVLGLCPCPGVMTVLLFLESFGLSENLFLFTLSMST-GIFLVILFFGILANTFKKTLVEDENF------KLHKIL 242 45556666678*****************9*99999999999998.88777777766676666654443222......234447 PP NicO 263 eivGavlvllfal 275 +vGa l +lf++ 23726_FusoPortal_Gene_1257 243 SLVGASLMILFGI 255 7777777777776 PP >> DUF2182 Predicted metal-binding integral membrane protein (DUF2182) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 10.5 0.0 9.3e-05 0.39 34 102 .. 92 161 .. 76 175 .. 0.82 2 ? 2.7 0.1 0.023 95 37 92 .. 205 258 .. 182 261 .. 0.78 Alignments for each domain: == domain 1 score: 10.5 bits; conditional E-value: 9.3e-05 DUF2182 34 aaalvlfvagYllvWlafglvatllqlllealallspllasassllaglvlllaglyqf.splkeaclkk 102 + +lv f+++Yl a+ lv ++++l +a+++l l+++ +l+a+++++l gly++ s l+++c ++ 23726_FusoPortal_Gene_1257 92 KLLLVSFLIAYLQGVSAYILVKFIINLSDKASMMLFYDLDNRTRLIASILIILIGLYNIySVLRNKCCEH 161 3478999999999999999999999999999999999999*****************9637789999877 PP == domain 2 score: 2.7 bits; conditional E-value: 0.023 DUF2182 37 lvlfvagYllvWlafglvatllqlllealallspllasassllaglvlllaglyqf 92 ++ + +g +lv l+fg++a ++ l + + l++ sl+++++++l g++q+ 23726_FusoPortal_Gene_1257 205 TLSMSTGIFLVILFFGILANTFKKTLVE--DENFKLHKILSLVGASLMILFGIFQI 258 5667789999999999999999888876..3444566779999999********96 PP >> DUF1980 Domain of unknown function (DUF1980) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 5.0 2.4 0.0046 19 7 57 .. 26 76 .. 20 108 .. 0.80 2 ? 12.9 0.1 1.8e-05 0.074 36 74 .. 136 172 .. 132 190 .. 0.77 3 ? 1.0 1.5 0.077 3.2e+02 34 88 .. 206 261 .. 200 263 .. 0.64 Alignments for each domain: == domain 1 score: 5.0 bits; conditional E-value: 0.0046 DUF1980 7 LlgFavllfkLhasgeiskYInpkyapllyfamvvllilalvqliraqkkk 57 L+++ + +++ + ++isk I ++++y+++++ ++ ++v+ + + + k 23726_FusoPortal_Gene_1257 26 LIMYKIAIYQQEIVEKISKLIEKENEKIIYTMLFFTFLYGIVHSFGPGHGK 76 8999999999999******************************99888887 PP == domain 2 score: 12.9 bits; conditional E-value: 1.8e-05 DUF1980 36 yfamvvllilalvqliraqkkkecghdHeeksfikklls 74 ++a++++++++l++++++ ++k+c+h+He +++k++l 23726_FusoPortal_Gene_1257 136 LIASILIILIGLYNIYSVLRNKCCEHHHE--TKVKNILG 172 58999*****************9999994..33333333 PP == domain 3 score: 1.0 bits; conditional E-value: 0.077 DUF1980 34 llyfamvvllilalvqliraqkkke.cghdHeeksfikkllsylilviPlllglll 88 l++ + ++l+il++ l + +kk+ ++ + + ++ ++ + + l++++ ++ +l+l 23726_FusoPortal_Gene_1257 206 LSMSTGIFLVILFFGILANTFKKTLvEDENFKLHKILSLVGASLMILFGIFQILIL 261 44455555555555556666666633555555777888888888888888777765 PP >> Zip ZIP Zinc transporter # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 4.6 11.4 0.0035 14 9 92 .. 175 261 .. 133 263 .. 0.80 Alignments for each domain: == domain 1 score: 4.6 bits; conditional E-value: 0.0035 Zip 9 iaililsllgllgpllvpkiskllsklllslllafavGvllgtaflhLlPealealeesqleee.hth...plagllvllGfl 87 i++ ++ g++ +ll+ s+ ls++l+ + l ++ G++l f+ +l+ +++ ++++ + h++ + a l++l+G++ 23726_FusoPortal_Gene_1257 175 IVLGLCPCPGVMTVLLFL-ESFGLSENLFLFTLSMSTGIFLVILFFGILANTFKKTLVEDENFKlHKIlslVGASLMILFGIF 256 333333333444444444.45559******************************988888888767779999999*******9 PP Zip 88 llllv 92 +l++ 23726_FusoPortal_Gene_1257 257 QILIL 261 99875 PP Internal pipeline statistics summary: ------------------------------------- Query sequence(s): 1 (264 residues searched) Target model(s): 16712 (2907032 nodes) Passed MSV filter: 1490 (0.0891575); expected 334.2 (0.02) Passed bias filter: 626 (0.0374581); expected 334.2 (0.02) Passed Vit filter: 35 (0.0020943); expected 16.7 (0.001) Passed Fwd filter: 7 (0.000418861); expected 0.2 (1e-05) Initial search space (Z): 16712 [actual number of targets] Domain search space (domZ): 4 [number of targets reported over threshold] # CPU time: 0.30u 0.25s 00:00:00.55 Elapsed: 00:00:00.33 # Mc/sec: 2325.63 // [ok]