# hmmscan :: search sequence(s) against a profile database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query sequence file: AA/23726_FusoPortal_Gene_1272.faa # target HMM database: Pfam-A.hmm # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: 23726_FusoPortal_Gene_1272 [L=449] Description: # 1389462 # 1390808 # -1 # ID=1_1272;partial=00;start_type=ATG;rbs_motif=GGA/GAG/AGG;rbs_spacer=5-10bp;gc_cont=0.252 Scores for complete sequence (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Model Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.7e-30 105.7 0.6 1e-29 103.1 0.0 2.5 3 AAA_14 AAA domain 3.7e-22 78.6 0.0 9.7e-22 77.3 0.1 1.8 2 DUF4143 Domain of unknown function (DUF4143) 0.0036 17.2 0.0 0.0082 16.0 0.0 1.6 1 AAA_5 AAA domain (dynein-related subfamily) ------ inclusion threshold ------ 0.055 13.3 5.8 4 7.2 0.2 3.5 3 ATPase_2 ATPase domain predominantly from Archaea 0.072 13.4 0.0 0.44 10.8 0.0 2.3 1 AAA_16 AAA ATPase domain 0.12 12.6 0.0 8.3 6.6 0.0 2.8 2 AAA_22 AAA domain Domain annotation for each model (and alignments): >> AAA_14 AAA domain # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 103.1 0.0 3.6e-33 1e-29 2 131 .] 18 152 .. 17 152 .. 0.89 2 ? -1.6 0.0 0.85 2.4e+03 66 99 .. 160 192 .. 154 197 .. 0.66 3 ? -1.6 0.2 0.84 2.3e+03 28 58 .. 371 401 .. 359 416 .. 0.71 Alignments for each domain: == domain 1 score: 103.1 bits; conditional E-value: 3.6e-33 AAA_14 2 rkilvitGaRrvGKTtllkqlikellvkkesilyinldderdkkla..dpekllelilek..lke.....ekktylflDEiqe 75 rk+l+++G+R+vGKT++lk++ + + e+i y+n+d+++++++ ++++++++++++ + + ++kt++++DE+q 23726_FusoPortal_Gene_1272 18 RKPLILKGVRQVGKTWILKEFGNICY---ENIAYFNFDENPEYRQFfqTTKDINRILQNLilI-SgykivPEKTLIIFDEVQD 96 689******************98888...**********999764421333777777776533.25889999*********** PP AAA_14 76 vpewekalkslvdegknldvivtGSnskllskeiatslagrvaeielyplsfaEfl 131 +pe+ + lk+++++++++++ ++GS++++ + + ++ ++g+v+ +++yp+sf+Efl 23726_FusoPortal_Gene_1272 97 APEVINSLKYFYENTPEYHITCAGSLLGISLAKPSSFPVGKVDFLNIYPMSFSEFL 152 *******************************************************7 PP == domain 2 score: -1.6 bits; conditional E-value: 0.85 AAA_14 66 tylflDEiqevpeweka.lkslvdegknldvivtG 99 lflD +++++ +a + l+++ + ++vtG 23726_FusoPortal_Gene_1272 160 LKLFLDNLNNIENIPDAfFNPLYEKL-K-MYYVTG 192 45899999999988665145555544.3.566666 PP == domain 3 score: -1.6 bits; conditional E-value: 0.84 AAA_14 28 vkkesilyinldderdkkladpekllelile 58 ++++i+ i+++ e++ k++ +k+ e +++ 23726_FusoPortal_Gene_1272 371 QNENNIIPIEVKAETNIKSKSLQKFKEKFKD 401 4567899999999888887766556655554 PP >> DUF4143 Domain of unknown function (DUF4143) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -3.1 0.0 2.8 7.8e+03 73 89 .. 1 17 [. 1 22 [. 0.69 2 ! 77.3 0.1 3.5e-25 9.7e-22 1 121 [. 222 383 .. 182 384 .. 0.93 Alignments for each domain: == domain 1 score: -3.1 bits; conditional E-value: 2.8 xxxxxxxxxxxxxxxxx RF DUF4143 73 lEnlVaqeLlkysekeg 89 +E +++++L+k++++++ 23726_FusoPortal_Gene_1272 1 MERFILNDLIKWKNSKY 17 68899999998755444 PP == domain 2 score: 77.3 bits; conditional E-value: 3.5e-25 xxxxxxxxxxxxxxxxxx...........................................xxxxxxxxxxx..xxxxxxxxx RF DUF4143 1 erDirklagvrdpeelrk...........................................glverlppwse..spnskkrlr 38 erD++k+ ++ e++k +lv +++++s+ +p+s++ ++ 23726_FusoPortal_Gene_1272 222 ERDFAKHPNI---YEFPKismiwksipsqlskenkkfiykvvkegarareyedalqwlvnaNLVTKVFKCSAprMPLSSYDDI 301 6788888888...6666677788899999999999999999999999***********************999********** PP xxxxxxxxxxxxxxxxxxxxxxxxxxxx....xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx RF DUF4143 39 kspKlyfvDtGLlvallgldkeelleds....enkGallEnlVaqeLlkysekegaklyyyrdkngaEvDfvielkggkivpi 117 +++K+y+vD+GLl+ l +l+++++ e++ e+kGal+En+++q L+ + ++ + y+ + n++Ev f+i+ ++++i+pi 23726_FusoPortal_Gene_1272 302 SAFKIYLVDVGLLTRLSQLSPNTFGEGNrlftEFKGALTENYILQGLIS---QFEVPPRYWAE-NNYEVYFIIQ-NENNIIPI 379 *************************999********************9...6799******9.69*******9.699***** PP xxxx RF DUF4143 118 EvKs 121 EvK+ 23726_FusoPortal_Gene_1272 380 EVKA 383 ***8 PP >> AAA_5 AAA domain (dynein-related subfamily) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 16.0 0.0 2.9e-06 0.0082 2 86 .. 21 105 .. 20 116 .. 0.91 Alignments for each domain: == domain 1 score: 16.0 bits; conditional E-value: 2.9e-06 EEEE--SSSSHHHHHHHGGGGBS--EEEEE--TT--HHHHH--B--.........--------...G-SEEEEESGGG--HHH CS AAA_5 2 vlLvGppGtgKselaerlaaalnreveyvqltrdtteedlkgrreikngttslvdsplvraar...egeilvlDEveraesev 81 ++L+G +gK+++ +++ ++ y ++ ++ +++++++ ++ + ++ l++ +l + e+ ++++DEv+ a +ev 23726_FusoPortal_Gene_1272 21 LILKGVRQVGKTWILKEFGNICYENIAYFNFDENPEYRQFFQTTK--DINRILQNLILISGYKivpEKTLIIFDEVQDA-PEV 100 89******************************************9..8999999999998888999***********55.789 PP HHHHH CS AAA_5 82 lnsLl 86 +nsL 23726_FusoPortal_Gene_1272 101 INSLK 105 99985 PP >> ATPase_2 ATPase domain predominantly from Archaea # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 7.2 0.2 0.0015 4 8 44 .. 6 42 .. 4 79 .. 0.76 2 ? 6.2 0.0 0.0028 7.9 118 197 .. 85 151 .. 56 201 .. 0.61 3 ? -0.0 0.9 0.23 6.4e+02 78 137 .. 379 441 .. 352 444 .. 0.57 Alignments for each domain: == domain 1 score: 7.2 bits; conditional E-value: 0.0015 ATPase_2 8 lkeleeilerepneiyfiYGpegsGKtaLlkeiienl 44 l++l ++ +++ ++++ + G +++GKt +lke+ + 23726_FusoPortal_Gene_1272 6 LNDLIKWKNSKYRKPLILKGVRQVGKTWILKEFGNIC 42 78999***************************98766 PP == domain 2 score: 6.2 bits; conditional E-value: 0.0028 ATPase_2 118 kkiiiiiDelqklidlglngaellvkeLlnliesltkekhlcvviclsSeglfiervyneselegrhkyilvddldketa 197 k ii+De+q + +++++L +++e+ t e+h++ + l +l +s +g+++++++ ++ + + 23726_FusoPortal_Gene_1272 85 EKTLIIFDEVQDAP--------EVINSLKYFYEN-TPEYHITCAGSLLGISLA----KPSSFPVGKVDFLNIYPMSFSEF 151 3579*******999........588888888888.666766555444444444....44999999999999999966443 PP == domain 3 score: -0.0 bits; conditional E-value: 0.23 ATPase_2 78 lekalgiavpkaeleeilkeksklvfkaielllekikkkgkk...iiiiiDelqklidlglng 137 +e ++ +++++++l+++ ++ + +++ + +e++k + + ++D + k+i++ ln+ 23726_FusoPortal_Gene_1272 379 IEVKAETNIKSKSLQKFKEKFKDDIKLRVRFSFENLKLDNDLlniPLFMVDYAEKIINIALNK 441 333444455555554433355566666666666666655544556777788888888887776 PP >> AAA_16 AAA ATPase domain # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 10.8 0.0 0.00016 0.44 25 88 .. 19 73 .. 5 181 .. 0.66 Alignments for each domain: == domain 1 score: 10.8 bits; conditional E-value: 0.00016 AAA_16 25 elvllsGesGvGKsslvrellralekdggiflrgkcdqlesaipysalaqalareellrqllde 88 ++l G + vGK+ +++e+ + ++ ++ ++ ++d++ +y ++ q+ + ++ +l++ 23726_FusoPortal_Gene_1272 19 KPLILKGVRQVGKTWILKEFGN-ICYEN--IAYFNFDEN---PEYRQFFQTT---KDINRILQN 73 67899****************6.55553..678888888...6677777777...456666555 PP >> AAA_22 AAA domain # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 6.6 0.0 0.003 8.3 4 28 .. 17 41 .. 13 125 .. 0.66 2 ? -3.0 0.0 2.7 7.4e+03 18 30 .. 388 400 .. 360 417 .. 0.71 Alignments for each domain: == domain 1 score: 6.6 bits; conditional E-value: 0.003 AAA_22 4 tagilvvtGesGtGKTtllrrlleq 28 +++ l++ G +GKT+ l+++ + 23726_FusoPortal_Gene_1272 17 YRKPLILKGVRQVGKTWILKEFGNI 41 5666777788888888888877664 PP == domain 2 score: -3.0 bits; conditional E-value: 2.7 AAA_22 18 KTtllrrlleqld 30 K+ +l++++e+++ 23726_FusoPortal_Gene_1272 388 KSKSLQKFKEKFK 400 5666666666654 PP Internal pipeline statistics summary: ------------------------------------- Query sequence(s): 1 (449 residues searched) Target model(s): 16712 (2907032 nodes) Passed MSV filter: 582 (0.0348253); expected 334.2 (0.02) Passed bias filter: 361 (0.0216012); expected 334.2 (0.02) Passed Vit filter: 39 (0.00233365); expected 16.7 (0.001) Passed Fwd filter: 6 (0.000359023); expected 0.2 (1e-05) Initial search space (Z): 16712 [actual number of targets] Domain search space (domZ): 6 [number of targets reported over threshold] # CPU time: 0.29u 0.23s 00:00:00.52 Elapsed: 00:00:00.33 # Mc/sec: 3955.33 // [ok]