# hmmscan :: search sequence(s) against a profile database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query sequence file: AA/23726_FusoPortal_Gene_1284.faa # target HMM database: Pfam-A.hmm # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: 23726_FusoPortal_Gene_1284 [L=291] Description: # 1401509 # 1402381 # -1 # ID=1_1284;partial=00;start_type=TTG;rbs_motif=GGAG/GAGG;rbs_spacer=5-10bp;gc_cont=0.260 Scores for complete sequence (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Model Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 9.7e-82 274.3 0.3 1.2e-81 274.1 0.3 1.0 1 ATP_bind_2 P-loop ATPase protein family 0.0039 17.6 0.1 0.012 16.1 0.1 1.8 1 AAA_18 AAA domain 0.0049 17.3 1.6 0.0083 16.6 1.5 1.5 1 AAA_23 AAA domain 0.0089 16.2 0.0 0.02 15.0 0.0 1.8 2 AAA_22 AAA domain ------ inclusion threshold ------ 0.011 16.1 0.2 0.028 14.9 0.1 1.8 2 ABC_tran ABC transporter 0.025 14.6 0.0 0.38 10.8 0.0 2.2 2 AAA_33 AAA domain 0.028 14.1 1.8 0.049 13.3 0.7 1.8 2 AAA_24 AAA domain 0.042 13.5 0.1 0.067 12.8 0.1 1.3 1 AAA_30 AAA domain 0.05 12.8 0.0 0.34 10.1 0.0 2.1 2 Zeta_toxin Zeta toxin 0.056 12.4 0.3 0.092 11.7 0.3 1.3 1 T2SSE Type II/IV secretion system protein 0.068 12.9 2.6 0.073 12.8 1.7 1.5 1 AAA_21 AAA domain, putative AbiEii toxin, Type IV TA sy 0.087 12.3 1.1 0.34 10.4 1.1 1.9 1 cobW CobW/HypB/UreG, nucleotide-binding domain 0.13 12.0 0.0 0.28 11.0 0.0 1.5 1 RsgA_GTPase RsgA GTPase 0.15 12.0 1.5 0.44 10.5 0.2 2.4 2 AAA_14 AAA domain Domain annotation for each model (and alignments): >> ATP_bind_2 P-loop ATPase protein family # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 274.1 0.3 9.6e-85 1.2e-81 2 273 .. 5 273 .. 4 287 .. 0.92 Alignments for each domain: == domain 1 score: 274.1 bits; conditional E-value: 9.6e-85 ATP_bind_2 2 elvivsGlsGaGksvalraledlGyyvvdnlPlsllpklvdllkeansreskvavvldvrnreesaelleqaeellekaitpr 84 +++iv+GlsGaGk++al+ led+ yy +dnlPl l +l+d+ k+av +d+r+ +++++ + + + e+ +++ 23726_FusoPortal_Gene_1284 5 HVIIVTGLSGAGKTTALNILEDMSYYTIDNLPLGLEKSLLDTEI------EKLAVGIDIRTFKNTKDFFTFINYIKESGVKMD 81 589**********************************9999876......89******************************* PP ATP_bind_2 85 ilfleadddtlvrryedtrrshPlsakdlslegiakerelleplraradlildtselsvrelreklrkalagnsekel....t 163 i+f+ea++++++ ry +rr hPl+ ++l +i ke+++l p+r+ adl++dt+e+++ el ++++k++ +++++++ + 23726_FusoPortal_Gene_1284 82 IIFIEAHEAIILGRYTLSRRAHPLKE-VTLLRSILKEKKILFPIREIADLVIDTTEIKTVELEKRFKKFIFAKDGENTdiniN 163 ************************86.56789************************************998888876611114 PP ATP_bind_2 164 vvvesfGfkyGiPldadlvfdvrflPnPhwveelrPltGkdkevaeyvlkrtevaefieklrsllslvlpslkregksyltia 246 + ++sfG+kyGiP d+dl+fdvrf+PnP+++e+l+ l G d+ev+eyvl+++e +ef kl ll++++p++ +egk++lti+ 23726_FusoPortal_Gene_1284 164 IHIQSFGYKYGIPTDSDLMFDVRFIPNPYYIEKLKELNGFDEEVKEYVLSQKESKEFYFKLLPLLEFLIPQYIKEGKKHLTIS 246 5679******************************************************************************* PP ATP_bind_2 247 iGctGGkhrsvaiaeqladyfrsdgkt 273 iGc GG+hrsv+ ++la+ +++ + + 23726_FusoPortal_Gene_1284 247 IGCSGGQHRSVTFVNKLAEDLKNSKVL 273 *******************99986543 PP >> AAA_18 AAA domain # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 16.1 0.1 9.7e-06 0.012 1 30 [. 7 33 .. 7 58 .. 0.83 Alignments for each domain: == domain 1 score: 16.1 bits; conditional E-value: 9.7e-06 AAA_18 1 IlltGtpGvGKttlareLskrlgfgvnlrd 30 I++tG +G+GKtt + L+++ ++ +d 23726_FusoPortal_Gene_1284 7 IIVTGLSGAGKTTALNILEDM---SYYTID 33 9*****************988...444444 PP >> AAA_23 AAA domain # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 16.6 1.5 7e-06 0.0083 19 175 .. 4 210 .. 3 270 .. 0.58 Alignments for each domain: == domain 1 score: 16.6 bits; conditional E-value: 7e-06 AAA_23 19 kgltlItGpNGaGKTTildAiklaLygetprlsrksgrgfeksdve...iese......gkvkayveltfenkdgry...... 86 k + ++tG GaGKTT l+ + + y +l+ + ++++ + ++e + + +++k + f+ ++ ++ 23726_FusoPortal_Gene_1284 4 KHVIIVTGLSGAGKTTALNILEDMSYYTIDNLPLGLEKSLLDTEIEklaV--GidirtfKNTKDF----FTFINYIKesgvkm 80 578999*********************99999999988888888875331..2222344222222....33333333333446 PP AAA_23 87 ......................trsiererekskkkgkttkkaklelleideleqfisellgskkiilnlilflgqer..... 142 + e + +s k+k++ + ++e+ + + +i++ ++ +k+ ++i+ ++ e+ 23726_FusoPortal_Gene_1284 81 diifieaheaiilgrytlsrraHPLKEVTLLRSILKEKKILFPIREIADLVIDTTEIKT-VELEKRFKKFIFAKDGENtdini 162 66666666677888666666552222224455555555555556666665555555554.44444444555555555533333 PP AAA_23 143 ....fekfelkdkke....................rleelekeleeledekkllekl 175 + + ++++l+el+ +++++++ 23726_FusoPortal_Gene_1284 163 nihiQ---------SfgykygiptdsdlmfdvrfiPNPYYIEKLKELNGFDEEVKEY 210 32220.........1444444444455555555542223444444444444444444 PP >> AAA_22 AAA domain # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 15.0 0.0 1.7e-05 0.02 4 28 .. 3 27 .. 1 122 [. 0.84 2 ? -2.2 0.0 3.7 4.4e+03 38 65 .. 240 268 .. 212 283 .. 0.66 Alignments for each domain: == domain 1 score: 15.0 bits; conditional E-value: 1.7e-05 AAA_22 4 tagilvvtGesGtGKTtllrrlleq 28 t+ +++vtG sG+GKTt l+ l + 23726_FusoPortal_Gene_1284 3 TKHVIIVTGLSGAGKTTALNILEDM 27 7899**************9988876 PP == domain 2 score: -2.2 bits; conditional E-value: 3.7 AAA_22 38 erpevvfvtlpsst.sskdllrellralg 65 ++ ++++ ++++ s ++ ++l+++l+ 23726_FusoPortal_Gene_1284 240 KKHLTISIGCSGGQhRSVTFVNKLAEDLK 268 44555556666655555566666666555 PP >> ABC_tran ABC transporter # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 14.9 0.1 2.3e-05 0.028 10 54 .. 3 48 .. 1 122 [. 0.79 2 ? -2.6 0.0 5.5 6.6e+03 109 124 .. 249 264 .. 228 268 .. 0.78 Alignments for each domain: == domain 1 score: 14.9 bits; conditional E-value: 2.3e-05 TTSEEEEEESTTSSHHHHHHHHTTSS--SEEEEEET.TEEGGGB-H CS xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx.xxxxxxxxx RF ABC_tran 10 egekvaivGenGaGKStLlkllagllkpteGeilld.gkdlkeqel 54 +++ ++G +GaGK+t l++l ++ t +++ l +k+l ++e+ 23726_FusoPortal_Gene_1284 3 TKHVIIVTGLSGAGKTTALNILEDMSYYTIDNLPLGlEKSLLDTEI 48 56788999**************999988877776555555555444 PP == domain 2 score: -2.6 bits; conditional E-value: 5.5 S-HHHHHHHHHHHHHH CS xxxxxxxxxxxxxxxx RF ABC_tran 109 LSgGqkqrvalarall 124 SgGq + v ++ l+ 23726_FusoPortal_Gene_1284 249 CSGGQHRSVTFVNKLA 264 5999999988877666 PP >> AAA_33 AAA domain # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 10.8 0.0 0.00031 0.38 1 17 [. 6 22 .. 6 34 .. 0.83 2 ? 1.4 0.0 0.26 3.1e+02 86 114 .. 67 95 .. 55 110 .. 0.82 Alignments for each domain: == domain 1 score: 10.8 bits; conditional E-value: 0.00031 AAA_33 1 lvllvGlpGsGKstlae 17 ++++ Gl+G+GK+t + 23726_FusoPortal_Gene_1284 6 VIIVTGLSGAGKTTALN 22 799***********875 PP == domain 2 score: 1.4 bits; conditional E-value: 0.26 AAA_33 86 arflklarrlgvpvrivvvetdeetlkeR 114 f++ ++ gv+ i+++e+ e+ + R 23726_FusoPortal_Gene_1284 67 FTFINYIKESGVKMDIIFIEAHEAIILGR 95 56889999***************999877 PP >> AAA_24 AAA domain # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 13.3 0.7 4.1e-05 0.049 2 72 .. 4 86 .. 3 93 .. 0.72 2 ? -0.8 0.1 0.85 1e+03 157 187 .. 121 154 .. 68 159 .. 0.63 Alignments for each domain: == domain 1 score: 13.3 bits; conditional E-value: 4.1e-05 AAA_24 2 klkiliyGpsgiGKTtlakel......gpkplfidtekGlksldadkfpdie.......swedfldevvelaaeladyktlviD 72 k i+++G sg+GKTt ++ l + + l + +ek+l +++ k+ + +df +++++++ + ++++i 23726_FusoPortal_Gene_1284 4 KHVIIVTGLSGAGKTTALNILedmsyyTIDNLPLGLEKSLLDTEIEKLAV-GidirtfkNTKDFFTFINYIKESGVKMDIIFIE 86 56799****************77766655666668888888877777542.124444446666666666669999999999986 PP == domain 2 score: -0.8 bits; conditional E-value: 0.85 AAA_24 157 anelkeevDlvlranlkred...kvgervlktsk 187 ++e++Dlv++ + + + k ++++++++ 23726_FusoPortal_Gene_1284 121 LFPIREIADLVIDTTEIKTVeleKRFKKFIFAKD 154 4457888888887654322246655555555555 PP >> AAA_30 AAA domain # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 12.8 0.1 5.6e-05 0.067 18 40 .. 4 26 .. 1 31 [. 0.84 Alignments for each domain: == domain 1 score: 12.8 bits; conditional E-value: 5.6e-05 AAA_30 18 drvavltGpAGtGKttalkalre 40 + v ++tG G+GKttal+ l++ 23726_FusoPortal_Gene_1284 4 KHVIIVTGLSGAGKTTALNILED 26 4599**************98865 PP >> Zeta_toxin Zeta toxin # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 10.1 0.0 0.00028 0.34 15 38 .. 3 26 .. 1 33 [. 0.88 2 ? 0.0 0.0 0.34 4e+02 102 135 .. 58 91 .. 54 157 .. 0.82 Alignments for each domain: == domain 1 score: 10.1 bits; conditional E-value: 0.00028 Zeta_toxin 15 rPklillgGqpGAGKselaellkd 38 ++ +i++ G +GAGK+++++ l+d 23726_FusoPortal_Gene_1284 3 TKHVIIVTGLSGAGKTTALNILED 26 6789***************99987 PP == domain 2 score: 0.0 bits; conditional E-value: 0.34 Zeta_toxin 102 gtlrspdvvkklaealkkaGYevevvvvavkkel 135 +t++++ ++ ++++ +k+ G ++ ++++ +++++ 23726_FusoPortal_Gene_1284 58 RTFKNTKDFFTFINYIKESGVKMDIIFIEAHEAI 91 5899999999999999999999999999888765 PP >> T2SSE Type II/IV secretion system protein # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 11.7 0.3 7.7e-05 0.092 128 149 .. 3 24 .. 1 31 [. 0.89 Alignments for each domain: == domain 1 score: 11.7 bits; conditional E-value: 7.7e-05 T2SSE 128 erglilvsGgTgsGKttllyal 149 ++ +i+v+G g GKtt l++l 23726_FusoPortal_Gene_1284 3 TKHVIIVTGLSGAGKTTALNIL 24 5789***************986 PP >> AAA_21 AAA domain, putative AbiEii toxin, Type IV TA system # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 12.8 1.7 6.1e-05 0.073 2 108 .. 7 124 .. 6 172 .. 0.76 Alignments for each domain: == domain 1 score: 12.8 bits; conditional E-value: 6.1e-05 AAA_21 2 nvliGpNgsGKSnLLdaldllarl...............drdvegltdelsggggirkipslldlydpeipfefeieeflsqg 69 +++G g+GK++ L +l+ ++ + + ++ ++++++k+ + + +y +e+ ++ i++ + ++ 23726_FusoPortal_Gene_1284 7 IIVTGLSGAGKTTALNILEDMSYYtidnlplglekslldTEIEKLAVGIDIRTFKNTKDFFTFINYIKESGVKMDIIFIEAHE 89 6899*****************999****9999999999877778888999999999999999999999999999999999999 PP AAA_21 70 vryryglrlersqveeklssvetllesgllldkrsleee 108 + ++++l r+++ + +e +l++ +l+ k++l+ + 23726_FusoPortal_Gene_1284 90 AIILGRYTLSRRAH----PLKEVTLLRSILKEKKILFPI 124 99999999999999....444444444444444444433 PP >> cobW CobW/HypB/UreG, nucleotide-binding domain # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 10.4 1.1 0.00029 0.34 2 20 .. 6 24 .. 5 91 .. 0.69 Alignments for each domain: == domain 1 score: 10.4 bits; conditional E-value: 0.00029 EEEEEESTTCCHHHHHHHH CS cobW 2 vtvltGflGsGKTtLlnhl 20 v+++tG G+GKTt ln l 23726_FusoPortal_Gene_1284 6 VIIVTGLSGAGKTTALNIL 24 899************9854 PP >> RsgA_GTPase RsgA GTPase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 11.0 0.0 0.00023 0.28 99 121 .. 4 26 .. 1 41 [. 0.86 Alignments for each domain: == domain 1 score: 11.0 bits; conditional E-value: 0.00023 TSEEEEECSTTSSHHHHHHHHCT CS RsgA_GTPase 99 dktsvlvGqSGvGKStLinallp 121 ++++++ G SG+GK+t +n l 23726_FusoPortal_Gene_1284 4 KHVIIVTGLSGAGKTTALNILED 26 58999**************9986 PP >> AAA_14 AAA domain # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 10.5 0.2 0.00037 0.44 2 97 .. 4 84 .. 3 106 .. 0.65 2 ? -2.8 0.1 4.6 5.5e+03 18 56 .. 110 150 .. 108 160 .. 0.52 Alignments for each domain: == domain 1 score: 10.5 bits; conditional E-value: 0.00037 AAA_14 2 rkilvitGaRrvGKTtllkqlikellvkkesilyinldderdkkladpekllelileklkeekktylflDEiqevpewekalksl 86 ++++++tG+ +GKTt l+ l e++ y+++d+ ++ ++ l+++i +++ + + + ++ ++++++++++ 23726_FusoPortal_Gene_1284 4 KHVIIVTGLSGAGKTTALNIL--------EDMSYYTIDNLPLGLEKSL--LDTEIEKLAVG-----IDIRTFKNTKDFFTFINYI 73 6799*************8544........4556777777776433322..34444443222.....3333556667777777777 PP AAA_14 87 vdegknldviv 97 ++g ++d+i+ 23726_FusoPortal_Gene_1284 74 KESGVKMDIIF 84 77776666666 PP == domain 2 score: -2.8 bits; conditional E-value: 4.6 AAA_14 18 llkqlikell..vkkesilyinldderdkkladpeklleli 56 ll+ ++ke+ + ++i + +d+ + k+ + ++++ ++i 23726_FusoPortal_Gene_1284 110 LLRSILKEKKilFPIREIADLVIDTTEIKTVELEKRFKKFI 150 55555555555555555555555555555444444444444 PP Internal pipeline statistics summary: ------------------------------------- Query sequence(s): 1 (291 residues searched) Target model(s): 16712 (2907032 nodes) Passed MSV filter: 614 (0.0367401); expected 334.2 (0.02) Passed bias filter: 412 (0.0246529); expected 334.2 (0.02) Passed Vit filter: 73 (0.00436812); expected 16.7 (0.001) Passed Fwd filter: 14 (0.000837721); expected 0.2 (1e-05) Initial search space (Z): 16712 [actual number of targets] Domain search space (domZ): 14 [number of targets reported over threshold] # CPU time: 0.29u 0.25s 00:00:00.54 Elapsed: 00:00:00.33 # Mc/sec: 2563.47 // [ok]