# hmmscan :: search sequence(s) against a profile database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query sequence file: AA/23726_FusoPortal_Gene_279.faa # target HMM database: Pfam-A.hmm # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: 23726_FusoPortal_Gene_279 [L=132] Description: # 297450 # 297845 # -1 # ID=1_279;partial=00;start_type=ATG;rbs_motif=AGGAGG;rbs_spacer=5-10bp;gc_cont=0.374 Scores for complete sequence (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Model Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.1e-06 26.9 0.1 5.2e-06 26.2 0.1 1.4 1 MliC Membrane-bound lysozyme-inhibitor of c-type lys ------ inclusion threshold ------ 0.038 13.7 0.4 0.054 13.2 0.4 1.3 1 PCYCGC Protein of unknown function with PCYCGC motif 0.13 12.5 0.1 0.21 11.8 0.1 1.4 1 MetallophosN N terminal of Calcineurin-like phosphoesterase 0.19 12.4 1.7 0.27 11.9 1.7 1.3 1 PCB_OB Penicillin-binding protein OB-like domain 3.9 8.0 8.1 9.7 6.8 8.1 1.9 1 OAD_gamma Oxaloacetate decarboxylase, gamma chain Domain annotation for each model (and alignments): >> MliC Membrane-bound lysozyme-inhibitor of c-type lysozyme # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 26.2 0.1 1.6e-09 5.2e-06 4 64 .. 60 122 .. 58 124 .. 0.83 Alignments for each domain: == domain 1 score: 26.2 bits; conditional E-value: 1.6e-09 CCCEEEEEEEECTTEEEEEEECCT.EEEEEEEEETSSSEEEE..ETEEEEEETTEEEEEETTTE CS MliC 4 dsgaevtvtyiagdpsfavlrlgg.ktltLaqavSaSGarYs..anyewWtkGdeatLtlggkd 64 ++g+e+t + +d s+a+l+ + k L++a +aSG+rY+ a+ e kG e++Ltlg+ + 23726_FusoPortal_Gene_279 60 EDGKEFTLVVA-ADGSTATLTDAEgKATELKNAETASGERYAdeAGNEVAMKGAEGILTLGDLK 122 67888888888.899*****98887999*************76526666699******998765 PP >> PCYCGC Protein of unknown function with PCYCGC motif # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 13.2 0.4 1.6e-05 0.054 3 52 .. 9 60 .. 7 81 .. 0.81 Alignments for each domain: == domain 1 score: 13.2 bits; conditional E-value: 1.6e-05 PCYCGC 3 lllalllsgCssdeaaeq..eadhaehsassgdiqeetasadvlPtfldeqd 52 l ++l l +C++++++e+ e++ +e +a+++ e+t++a ++ tf +++ 23726_FusoPortal_Gene_279 9 LAMSLFLVACGEKKEEEKpaEQAAVEATATEAPATETTEAAAEAKTFSLKTE 60 67899********999987677778888899*************99955544 PP >> MetallophosN N terminal of Calcineurin-like phosphoesterase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 11.8 0.1 6.3e-05 0.21 5 29 .. 60 84 .. 56 96 .. 0.84 Alignments for each domain: == domain 1 score: 11.8 bits; conditional E-value: 6.3e-05 MetallophosN 5 skgkgvagVvVsDGvnvtkTdakGr 29 ++gk + Vv DG + t Tda+G+ 23726_FusoPortal_Gene_279 60 EDGKEFTLVVAADGSTATLTDAEGK 84 78999999****************8 PP >> PCB_OB Penicillin-binding protein OB-like domain # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 11.9 1.7 8e-05 0.27 11 92 .. 40 121 .. 20 129 .. 0.86 Alignments for each domain: == domain 1 score: 11.9 bits; conditional E-value: 8e-05 -SHCT..HHHHHHHCCTSSTCTTEEEEEEEEEETTEEEEEETTS-EEEEEGGGGTTTGCCTTTS-B---CCCHCCC--TTEE CS PCB_OB 11 ealaeadeealeealkklpdsgelrpAvVlsvseksarvglrdgetgtipleglkwarkvlsdkkqgkapksasdvlkpGdv 92 + +e+ e+a+e++ +l+++++ + +V++++ + a++ ++g+ +++ ++ + +++ ++ + a k a+ +l+ Gd+ 23726_FusoPortal_Gene_279 40 APATETTEAAAEAKTFSLKTEDGKEFTLVVAADGSTATLTDAEGKATELKNAETASGERYADEAGNEVAMKGAEGILTLGDL 121 3334455667777777888999**********************************************************97 PP >> OAD_gamma Oxaloacetate decarboxylase, gamma chain # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 6.8 8.1 0.0029 9.7 20 58 .. 6 53 .. 4 109 .. 0.78 Alignments for each domain: == domain 1 score: 6.8 bits; conditional E-value: 0.0029 OAD_gamma 20 lLslvislfskiikkkakkkkkkakaaaaaaaaaaaaa.........e 58 +L l++slf k++++k++++aa +a+a++a+a+ + 23726_FusoPortal_Gene_279 6 MLALAMSLFLVACGEKKEEEKPAEQAAVEATATEAPATetteaaaeaK 53 799999999999988888888888888777777766655555555550 PP Internal pipeline statistics summary: ------------------------------------- Query sequence(s): 1 (132 residues searched) Target model(s): 16712 (2907032 nodes) Passed MSV filter: 1246 (0.0745572); expected 334.2 (0.02) Passed bias filter: 704 (0.0421254); expected 334.2 (0.02) Passed Vit filter: 70 (0.00418861); expected 16.7 (0.001) Passed Fwd filter: 5 (0.000299186); expected 0.2 (1e-05) Initial search space (Z): 16712 [actual number of targets] Domain search space (domZ): 5 [number of targets reported over threshold] # CPU time: 0.25u 0.24s 00:00:00.49 Elapsed: 00:00:00.32 # Mc/sec: 1199.15 // [ok]