# hmmscan :: search sequence(s) against a profile database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query sequence file: AA/23726_FusoPortal_Gene_695.faa # target HMM database: Pfam-A.hmm # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: 23726_FusoPortal_Gene_695 [L=208] Description: # 786912 # 787535 # 1 # ID=1_695;partial=00;start_type=ATG;rbs_motif=GGAG/GAGG;rbs_spacer=5-10bp;gc_cont=0.269 Scores for complete sequence (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Model Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.3e-29 102.2 14.0 2.6e-29 102.0 14.0 1.0 1 LysE LysE type translocator ------ inclusion threshold ------ 0.082 12.9 0.3 0.082 12.9 0.3 2.4 2 Phage_holin_3_6 Putative Actinobacterial Holin-X, holin supe 4.9 7.0 11.9 0.25 11.2 1.4 2.4 2 DctQ Tripartite ATP-independent periplasmic trans Domain annotation for each model (and alignments): >> LysE LysE type translocator # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 102.0 14.0 4.7e-33 2.6e-29 3 191 .. 14 196 .. 12 198 .. 0.94 Alignments for each domain: == domain 1 score: 102.0 bits; conditional E-value: 4.7e-33 LysE 3 lllspGpqnllvlkqgikrgfkggilvalgvligdavlillgllGlalLlkaapivlsilrllGgayLlwlgvlalrdakekkv 86 ++++G qnl+v++ +i++ + +++l+al v+ d+ l+++ +G+ lL+++ +++ i+ l+G ++ +++g lr++ e k 23726_FusoPortal_Gene_695 14 YVAPIGVQNLFVINSAITQKRSKALLIALIVIFFDVTLAFACFFGIGLLIDKLEWLKLIILLVGSLVIIYIGQGLLRSKSELKK 97 5789**************************************************************************999885 PP LysE 87 eaeksveaslvkpflkglllslsNPkailfwlsvisallakaeqygdagrlilaaglvvasliwfsllaflaarlsrrlsskkv 170 + + l k + ++ ++++ NP+ai++ +++a+ a + +++ + + +g+++as++wf+ l+++ +++s++++ k 23726_FusoPortal_Gene_695 98 N-DNMDI-PLLKAITSACVVTWFNPQAIIDGTMMLGAFRA---TLSSEAGIYFILGVTSASFCWFMGLSIFISLFSHKFNDKV- 175 3.34443.8*******************************...89999999999**************************995. PP LysE 171 lriinvvvgvlligfavklll 191 l++in+v+g+++i+ +vklll 23726_FusoPortal_Gene_695 176 LKVINIVCGLVIIFYGVKLLL 196 77*****************98 PP >> Phage_holin_3_6 Putative Actinobacterial Holin-X, holin superfamily III # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 12.9 0.3 1.5e-05 0.082 37 94 .. 37 98 .. 28 104 .. 0.58 2 ? 2.0 2.1 0.034 1.9e+02 43 90 .. 141 194 .. 124 202 .. 0.68 Alignments for each domain: == domain 1 score: 12.9 bits; conditional E-value: 1.5e-05 Phage_holin_3_6 37 agllgaalvlallalllllvala.lala.alwlaali..vaavllvlagvlalvglkklkka 94 a+l+++ ++++ ++l++++++ + l+++ + wl +i v+ ++++ g +l+ +++lkk+ 23726_FusoPortal_Gene_695 37 ALLIALIVIFFDVTLAFACFFGIgLLIDkLEWLKLIIllVGSLVIIYIGQGLLRSKSELKKN 98 34444444444444444444443255554488888773255556666677777777788875 PP == domain 2 score: 2.0 bits; conditional E-value: 0.034 Phage_holin_3_6 43 alvlallalllllvalalala.a.....lwlaalivaavllvlagvlalvglkk 90 a++ ++l++++++++ +++l+ + + ++ ++ +v ++v ++ g+k 23726_FusoPortal_Gene_695 141 AGIYFILGVTSASFCWFMGLSiFislfsHKFNDKVLKVINIVCGLVIIFYGVKL 194 566666667776666666664334444656666666788888888888777774 PP >> DctQ Tripartite ATP-independent periplasmic transporters, DctQ component # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 1.1 3.4 0.059 3.3e+02 22 74 .. 31 82 .. 10 118 .. 0.74 2 ? 11.2 1.4 4.4e-05 0.25 37 85 .. 151 199 .. 128 207 .. 0.76 Alignments for each domain: == domain 1 score: 1.1 bits; conditional E-value: 0.059 DctQ 22 teelarylfvwlvflgaalalrrgshirvdllverlpprvrrlleliadllal 74 t+ +++l+++l+ +++ ++l + + ll+++l ++++ ++ l++ l+++ 23726_FusoPortal_Gene_695 31 TQKRSKALLIALIVIFFDVTLAFACFFGIGLLIDKL-EWLKLIILLVGSLVII 82 556677888888888888888888888888888888.5555555555555554 PP == domain 2 score: 11.2 bits; conditional E-value: 4.4e-05 DctQ 37 gaalalrrgshirvdllverlpprvrrlleliadllallfaavliwagw 85 +a+ + g i + l+ ++++ +v +++++++ l+++++++ l+ + 23726_FusoPortal_Gene_695 151 SASFCWFMGLSIFISLFSHKFNDKVLKVINIVCGLVIIFYGVKLLLNFY 199 5555666678899**************************9985554444 PP Internal pipeline statistics summary: ------------------------------------- Query sequence(s): 1 (208 residues searched) Target model(s): 16712 (2907032 nodes) Passed MSV filter: 1006 (0.0601963); expected 334.2 (0.02) Passed bias filter: 346 (0.0207037); expected 334.2 (0.02) Passed Vit filter: 31 (0.00185495); expected 16.7 (0.001) Passed Fwd filter: 8 (0.000478698); expected 0.2 (1e-05) Initial search space (Z): 16712 [actual number of targets] Domain search space (domZ): 3 [number of targets reported over threshold] # CPU time: 0.25u 0.24s 00:00:00.49 Elapsed: 00:00:00.31 # Mc/sec: 1950.52 // [ok]