1_1_36S_FusoPortal_Gene_1848

Protein

1_1_36S_FusoPortal_Gene_1848

Length

931 amino acids

Protein family membership

Isoleucine-tRNA ligase (IPR002301)
- Isoleucine-tRNA ligase, type 1 (IPR023585)

Homologous superfamilies

Homologous superfamily
43 - 122
Rossmann-like alpha/beta/alpha sandwich fold (IPR014729)
203 - 497
Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain (IPR009008)
653 - 931
Aminoacyl-tRNA synthetase, class Ia, anticodon-binding (IPR009080)

1 100 200 300 400 500 600 700 800 931

Domains and repeats

Domain
31 - 642
Aminoacyl-tRNA synthetase, class Ia (IPR002300)
Domain
641 - 829
Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding (IPR013155)
Isoleucyl tRNA synthetase type 1, anticodon-binding domain (IPR033708)
900 - 928
Zinc finger, FPG/IleRS-type (IPR010663)

1 100 200 300 400 500 600 700 800 931

Detailed signature matches

IPR014729

Rossmann-like alpha/beta/alpha sandwich fold
1. G3DSA:3.40.50...
  
  43 - 122
  
  GENE3D
  G3DSA:3.40.50.620 (G3DSA:3.40.50.620)
IPR009008

Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain
1. G3DSA:3.90.74...
  
  205 - 497
  
  GENE3D
  G3DSA:3.90.740.10 (G3DSA:3.90.740.10)
2. SSF50677 (ValRS/Ile...)
  
  203 - 398
  
  SUPERFAMILY
  SSF50677 (ValRS/IleRS/LeuRS editing domain)
IPR009080

Aminoacyl-tRNA synthetase, class Ia, anticodon-binding
1. SSF47323 (Anticodon...)
  
  653 - 931
  
  SUPERFAMILY
  SSF47323 (Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases)
IPR002301

Isoleucine-tRNA ligase
1. TIGR00392
  
  17 - 844
  
  TIGRFAMs
  TIGR00392 (TIGR00392)
2. PR00984 (TRNASYNTHILE)
  
  51 - 62
  
  PRINTS
  PR00984 (TRNASYNTHILE)
  
  233 - 256
  
  PRINTS
  PR00984 (TRNASYNTHILE)
  
  562 - 571
  
  PRINTS
  PR00984 (TRNASYNTHILE)
  
  528 - 541
  
  PRINTS
  PR00984 (TRNASYNTHILE)
  
  396 - 411
  
  PRINTS
  PR00984 (TRNASYNTHILE)
IPR023585

Isoleucine-tRNA ligase, type 1
1. MF_02002 (Ile_tRNA_...)
  
  4 - 931
  
  HAMAP
  MF_02002 (Ile_tRNA_synth_type1)
IPR002300

Aminoacyl-tRNA synthetase, class Ia
1. PF00133 (tRNA-synt_1)
  
  31 - 642
  
  Pfam
  PF00133 (tRNA-synt_1)
IPR013155

Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding
1. PF08264 (Anticodon_1)
  
  686 - 829
  
  Pfam
  PF08264 (Anticodon_1)
IPR033708

Isoleucyl tRNA synthetase type 1, anticodon-binding domain
1. cd07960 (Anticodon_...)
  
  641 - 821
  
  CDD
  cd07960 (Anticodon_Ia_Ile_BEm)
IPR010663

Zinc finger, FPG/IleRS-type
1. PF06827 (zf-FPG_IleRS)
  
  900 - 928
  
  Pfam
  PF06827 (zf-FPG_IleRS)

no IPR

Unintegrated signatures

Coil

813 - 833

COILS
Coil (Coil)
G3DSA:1.10.73...

588 - 815

GENE3D
G3DSA:1.10.730.10 (G3DSA:1.10.730.10)
G3DSA:3.30.23...

816 - 898

GENE3D
G3DSA:3.30.2320.20 (G3DSA:3.30.2320.20)
PTHR42765 (FAMILY N...)

3 - 930

PANTHER
PTHR42765 (FAMILY NOT NAMED)
PTHR42765:SF1 (ISOL...)

3 - 930

PANTHER
PTHR42765:SF1 (ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL)
SSF52374 (Nucleotid...)

6 - 202

SUPERFAMILY
SSF52374 (Nucleotidylyl transferase)

398 - 648

SUPERFAMILY
SSF52374 (Nucleotidylyl transferase)
cd00818 (IleRS_core)

49 - 641

CDD
cd00818 (IleRS_core)

Residue annotation

active site cd00818

57P

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

58P

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

59Y

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

65H

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

67G

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

68H

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

71N

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

446R

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

536W

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

539S

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

562E

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

563G

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

564S

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

565D

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

566Q

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

568R

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

570W

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

593H

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

594G

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

595F

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

596V

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

597N

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

601G

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

602E

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

603K

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

604M

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

605S

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

606K

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)

638D

active site
57P, 58P, 59Y, 65H, 67G, 68H, 71N, 446R, 536W, 539S, 562E, 563G, 564S, 565D, 566Q, 568R, 570W, 593H, 594G, 595F, 596V, 597N, 601G, 602E, 603K, 604M, 605S, 606K, 638D
CDD
cd00818 (IleRS_core)
HIGH motif cd00818

65H

HIGH motif
65H, 66I, 67G, 68H
CDD
cd00818 (IleRS_core)

66I

HIGH motif
65H, 66I, 67G, 68H
CDD
cd00818 (IleRS_core)

67G

HIGH motif
65H, 66I, 67G, 68H
CDD
cd00818 (IleRS_core)

68H

HIGH motif
65H, 66I, 67G, 68H
CDD
cd00818 (IleRS_core)
KMSKS motif cd00818

603K

KMSKS motif
603K, 604M, 605S, 606K, 607S
CDD
cd00818 (IleRS_core)

604M

KMSKS motif
603K, 604M, 605S, 606K, 607S
CDD
cd00818 (IleRS_core)

605S

KMSKS motif
603K, 604M, 605S, 606K, 607S
CDD
cd00818 (IleRS_core)

606K

KMSKS motif
603K, 604M, 605S, 606K, 607S
CDD
cd00818 (IleRS_core)

607S

KMSKS motif
603K, 604M, 605S, 606K, 607S
CDD
cd00818 (IleRS_core)
tRNA binding surfa...

644N

tRNA binding surface
644N, 648Q, 651E, 655R, 658N, 661R, 662Y, 710Y, 713F, 714Q, 717H, 718Y, 721G, 726A, 730D, 733K, 818K, 821N
CDD
cd07960 (Anticodon_Ia_Ile_BEm)

648Q

tRNA binding surface
644N, 648Q, 651E, 655R, 658N, 661R, 662Y, 710Y, 713F, 714Q, 717H, 718Y, 721G, 726A, 730D, 733K, 818K, 821N
CDD
cd07960 (Anticodon_Ia_Ile_BEm)

651E

tRNA binding surface
644N, 648Q, 651E, 655R, 658N, 661R, 662Y, 710Y, 713F, 714Q, 717H, 718Y, 721G, 726A, 730D, 733K, 818K, 821N
CDD
cd07960 (Anticodon_Ia_Ile_BEm)

655R

tRNA binding surface
644N, 648Q, 651E, 655R, 658N, 661R, 662Y, 710Y, 713F, 714Q, 717H, 718Y, 721G, 726A, 730D, 733K, 818K, 821N
CDD
cd07960 (Anticodon_Ia_Ile_BEm)

658N

tRNA binding surface
644N, 648Q, 651E, 655R, 658N, 661R, 662Y, 710Y, 713F, 714Q, 717H, 718Y, 721G, 726A, 730D, 733K, 818K, 821N
CDD
cd07960 (Anticodon_Ia_Ile_BEm)

661R

tRNA binding surface
644N, 648Q, 651E, 655R, 658N, 661R, 662Y, 710Y, 713F, 714Q, 717H, 718Y, 721G, 726A, 730D, 733K, 818K, 821N
CDD
cd07960 (Anticodon_Ia_Ile_BEm)

662Y

tRNA binding surface
644N, 648Q, 651E, 655R, 658N, 661R, 662Y, 710Y, 713F, 714Q, 717H, 718Y, 721G, 726A, 730D, 733K, 818K, 821N
CDD
cd07960 (Anticodon_Ia_Ile_BEm)

710Y

tRNA binding surface
644N, 648Q, 651E, 655R, 658N, 661R, 662Y, 710Y, 713F, 714Q, 717H, 718Y, 721G, 726A, 730D, 733K, 818K, 821N
CDD
cd07960 (Anticodon_Ia_Ile_BEm)

713F

tRNA binding surface
644N, 648Q, 651E, 655R, 658N, 661R, 662Y, 710Y, 713F, 714Q, 717H, 718Y, 721G, 726A, 730D, 733K, 818K, 821N
CDD
cd07960 (Anticodon_Ia_Ile_BEm)

714Q

tRNA binding surface
644N, 648Q, 651E, 655R, 658N, 661R, 662Y, 710Y, 713F, 714Q, 717H, 718Y, 721G, 726A, 730D, 733K, 818K, 821N
CDD
cd07960 (Anticodon_Ia_Ile_BEm)

717H

tRNA binding surface
644N, 648Q, 651E, 655R, 658N, 661R, 662Y, 710Y, 713F, 714Q, 717H, 718Y, 721G, 726A, 730D, 733K, 818K, 821N
CDD
cd07960 (Anticodon_Ia_Ile_BEm)

718Y

tRNA binding surface
644N, 648Q, 651E, 655R, 658N, 661R, 662Y, 710Y, 713F, 714Q, 717H, 718Y, 721G, 726A, 730D, 733K, 818K, 821N
CDD
cd07960 (Anticodon_Ia_Ile_BEm)

721G

tRNA binding surface
644N, 648Q, 651E, 655R, 658N, 661R, 662Y, 710Y, 713F, 714Q, 717H, 718Y, 721G, 726A, 730D, 733K, 818K, 821N
CDD
cd07960 (Anticodon_Ia_Ile_BEm)

726A

tRNA binding surface
644N, 648Q, 651E, 655R, 658N, 661R, 662Y, 710Y, 713F, 714Q, 717H, 718Y, 721G, 726A, 730D, 733K, 818K, 821N
CDD
cd07960 (Anticodon_Ia_Ile_BEm)

730D

tRNA binding surface
644N, 648Q, 651E, 655R, 658N, 661R, 662Y, 710Y, 713F, 714Q, 717H, 718Y, 721G, 726A, 730D, 733K, 818K, 821N
CDD
cd07960 (Anticodon_Ia_Ile_BEm)

733K

tRNA binding surface
644N, 648Q, 651E, 655R, 658N, 661R, 662Y, 710Y, 713F, 714Q, 717H, 718Y, 721G, 726A, 730D, 733K, 818K, 821N
CDD
cd07960 (Anticodon_Ia_Ile_BEm)

818K

tRNA binding surface
644N, 648Q, 651E, 655R, 658N, 661R, 662Y, 710Y, 713F, 714Q, 717H, 718Y, 721G, 726A, 730D, 733K, 818K, 821N
CDD
cd07960 (Anticodon_Ia_Ile_BEm)

821N

tRNA binding surface
644N, 648Q, 651E, 655R, 658N, 661R, 662Y, 710Y, 713F, 714Q, 717H, 718Y, 721G, 726A, 730D, 733K, 818K, 821N
CDD
cd07960 (Anticodon_Ia_Ile_BEm)
anticodon binding ...

658N

anticodon binding site
658N, 661R
CDD
cd07960 (Anticodon_Ia_Ile_BEm)

661R

anticodon binding site
658N, 661R
CDD
cd07960 (Anticodon_Ia_Ile_BEm)

GO term prediction

Biological Process

GO:0006418 tRNA aminoacylation for protein translation
GO:0006428 isoleucyl-tRNA aminoacylation

Molecular Function

GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0002161 aminoacyl-tRNA editing activity
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004822 isoleucine-tRNA ligase activity
GO:0005524 ATP binding

Cellular Component

GO:0005737 cytoplasm

Filter view on

Entry type

Status

Per-residue features

1_1_36S_FusoPortal_Gene_1848

Protein family membership

Homologous superfamilies

Domains and repeats

Detailed signature matches

Residue annotation

GO term prediction

Biological Process

Molecular Function

Cellular Component