25586_FusoPortal_Gene_1804

Protein

25586_FusoPortal_Gene_1804

Length

636 amino acids

Protein family membership

Methionyl-tRNA synthetase (IPR014758)
- Methionine-tRNA synthetase, type 2 (IPR023457)

Homologous superfamilies

Homologous superfamily
2 - 345
Rossmann-like alpha/beta/alpha sandwich fold (IPR014729)
354 - 520
Aminoacyl-tRNA synthetase, class Ia, anticodon-binding (IPR009080)
535 - 633
Nucleic acid-binding, OB-fold (IPR012340)

1 100 200 300 400 500 636

Domains and repeats

Domain
4 - 362
Methionyl/Leucyl tRNA synthetase (IPR015413)
Methioninyl-tRNA synthetase core domain (IPR033911)
383 - 520
Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding (IPR013155)
531 - 636
tRNA-binding domain (IPR002547)
Methionyl-tRNA synthetase, beta subunit, C-terminal (IPR004495)

1 100 200 300 400 500 636

Detailed signature matches

IPR014729

Rossmann-like alpha/beta/alpha sandwich fold
1. G3DSA:3.40.50...
  
  2 - 345
  
  GENE3D
  G3DSA:3.40.50.620 (G3DSA:3.40.50.620)
IPR009080

Aminoacyl-tRNA synthetase, class Ia, anticodon-binding
1. SSF47323 (Anticodon...)
  
  354 - 520
  
  SUPERFAMILY
  SSF47323 (Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases)
IPR012340

Nucleic acid-binding, OB-fold
1. SSF50249 (Nucleic a...)
  
  535 - 633
  
  SUPERFAMILY
  SSF50249 (Nucleic acid-binding proteins)
IPR014758

Methionyl-tRNA synthetase
1. TIGR00398
  
  6 - 512
  
  TIGRFAMs
  TIGR00398 (TIGR00398)
IPR023457

Methionine-tRNA synthetase, type 2
1. MF_01228 (Met_tRNA_...)
  
  1 - 510
  
  HAMAP
  MF_01228 (Met_tRNA_synth_type2)
IPR015413

Methionyl/Leucyl tRNA synthetase
1. PF09334 (tRNA-synt_1g)
  
  6 - 362
  
  Pfam
  PF09334 (tRNA-synt_1g)
IPR033911

Methioninyl-tRNA synthetase core domain
1. PR01041 (TRNASYNTHMET)
  
  7 - 20
  
  PRINTS
  PR01041 (TRNASYNTHMET)
  
  349 - 360
  
  PRINTS
  PR01041 (TRNASYNTHMET)
  
  39 - 53
  
  PRINTS
  PR01041 (TRNASYNTHMET)
  
  223 - 234
  
  PRINTS
  PR01041 (TRNASYNTHMET)
  
  87 - 98
  
  PRINTS
  PR01041 (TRNASYNTHMET)
  
  257 - 272
  
  PRINTS
  PR01041 (TRNASYNTHMET)
2. cd00814 (MetRS_core)
  
  4 - 337
  
  CDD
  cd00814 (MetRS_core)
IPR013155

Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding
1. PF08264 (Anticodon_1)
  
  383 - 520
  
  Pfam
  PF08264 (Anticodon_1)
IPR002547

tRNA-binding domain
1. PF01588 (tRNA_bind)
  
  541 - 633
  
  Pfam
  PF01588 (tRNA_bind)
2. PS50886 (TRBD)
  
  535 - 636
  
  PROSITE profiles
  PS50886 (TRBD)
IPR004495

Methionyl-tRNA synthetase, beta subunit, C-terminal
1. cd02800 (tRNA_bind_...)
  
  531 - 636
  
  CDD
  cd02800 (tRNA_bind_EcMetRS_like)

no IPR

Unintegrated signatures

G3DSA:1.10.73...

346 - 513

GENE3D
G3DSA:1.10.730.10 (G3DSA:1.10.730.10)
G3DSA:2.40.50...

534 - 636

GENE3D
G3DSA:2.40.50.140 (G3DSA:2.40.50.140)
PTHR43326 (FAMILY N...)

2 - 522

PANTHER
PTHR43326 (FAMILY NOT NAMED)
PTHR43326:SF3 (SUBF...)

2 - 522

PANTHER
PTHR43326:SF3 (SUBFAMILY NOT NAMED)
SSF52374 (Nucleotid...)

2 - 352

SUPERFAMILY
SSF52374 (Nucleotidylyl transferase)
cd07957 (Anticodon_...)

346 - 475

CDD
cd07957 (Anticodon_Ia_Met)

Residue annotation

active site cd00814

10P

active site
10P, 11I, 13Y, 50D, 226W, 229A, 230L, 233Y, 263I, 267H
CDD
cd00814 (MetRS_core)

11I

active site
10P, 11I, 13Y, 50D, 226W, 229A, 230L, 233Y, 263I, 267H
CDD
cd00814 (MetRS_core)

13Y

active site
10P, 11I, 13Y, 50D, 226W, 229A, 230L, 233Y, 263I, 267H
CDD
cd00814 (MetRS_core)

50D

active site
10P, 11I, 13Y, 50D, 226W, 229A, 230L, 233Y, 263I, 267H
CDD
cd00814 (MetRS_core)

226W

active site
10P, 11I, 13Y, 50D, 226W, 229A, 230L, 233Y, 263I, 267H
CDD
cd00814 (MetRS_core)

229A

active site
10P, 11I, 13Y, 50D, 226W, 229A, 230L, 233Y, 263I, 267H
CDD
cd00814 (MetRS_core)

230L

active site
10P, 11I, 13Y, 50D, 226W, 229A, 230L, 233Y, 263I, 267H
CDD
cd00814 (MetRS_core)

233Y

active site
10P, 11I, 13Y, 50D, 226W, 229A, 230L, 233Y, 263I, 267H
CDD
cd00814 (MetRS_core)

263I

active site
10P, 11I, 13Y, 50D, 226W, 229A, 230L, 233Y, 263I, 267H
CDD
cd00814 (MetRS_core)

267H

active site
10P, 11I, 13Y, 50D, 226W, 229A, 230L, 233Y, 263I, 267H
CDD
cd00814 (MetRS_core)
HIGH motif cd00814

19H

HIGH motif
19H, 20V, 21G, 22S
CDD
cd00814 (MetRS_core)

20V

HIGH motif
19H, 20V, 21G, 22S
CDD
cd00814 (MetRS_core)

21G

HIGH motif
19H, 20V, 21G, 22S
CDD
cd00814 (MetRS_core)

22S

HIGH motif
19H, 20V, 21G, 22S
CDD
cd00814 (MetRS_core)
KMSKS motif cd00814

298K

KMSKS motif
298K, 299M, 300S, 301K, 302S
CDD
cd00814 (MetRS_core)

299M

KMSKS motif
298K, 299M, 300S, 301K, 302S
CDD
cd00814 (MetRS_core)

300S

KMSKS motif
298K, 299M, 300S, 301K, 302S
CDD
cd00814 (MetRS_core)

301K

KMSKS motif
298K, 299M, 300S, 301K, 302S
CDD
cd00814 (MetRS_core)

302S

KMSKS motif
298K, 299M, 300S, 301K, 302S
CDD
cd00814 (MetRS_core)
tRNA binding surfa...

347N

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

354L

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

355G

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

358L

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

359N

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

362L

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

363G

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

366K

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

367K

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

370N

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

371G

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

421N

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

422K

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

424I

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

425D

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

430W

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)
anticodon binding ...

354L

anticodon binding site
354L, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 424I, 430W
CDD
cd07957 (Anticodon_Ia_Met)

358L

anticodon binding site
354L, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 424I, 430W
CDD
cd07957 (Anticodon_Ia_Met)

359N

anticodon binding site
354L, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 424I, 430W
CDD
cd07957 (Anticodon_Ia_Met)

362L

anticodon binding site
354L, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 424I, 430W
CDD
cd07957 (Anticodon_Ia_Met)

363G

anticodon binding site
354L, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 424I, 430W
CDD
cd07957 (Anticodon_Ia_Met)

366K

anticodon binding site
354L, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 424I, 430W
CDD
cd07957 (Anticodon_Ia_Met)

367K

anticodon binding site
354L, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 424I, 430W
CDD
cd07957 (Anticodon_Ia_Met)

370N

anticodon binding site
354L, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 424I, 430W
CDD
cd07957 (Anticodon_Ia_Met)

371G

anticodon binding site
354L, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 424I, 430W
CDD
cd07957 (Anticodon_Ia_Met)

424I

anticodon binding site
354L, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 424I, 430W
CDD
cd07957 (Anticodon_Ia_Met)

430W

anticodon binding site
354L, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 424I, 430W
CDD
cd07957 (Anticodon_Ia_Met)
dimer interface cd...

532D

dimer interface
532D, 579F, 596N, 597L, 598K, 601K, 613T, 614T, 615E, 616D, 622L, 624K, 626D, 629V, 633A, 634I, 635V, 636S
CDD
cd02800 (tRNA_bind_EcMetRS_like)

579F

dimer interface
532D, 579F, 596N, 597L, 598K, 601K, 613T, 614T, 615E, 616D, 622L, 624K, 626D, 629V, 633A, 634I, 635V, 636S
CDD
cd02800 (tRNA_bind_EcMetRS_like)

596N

dimer interface
532D, 579F, 596N, 597L, 598K, 601K, 613T, 614T, 615E, 616D, 622L, 624K, 626D, 629V, 633A, 634I, 635V, 636S
CDD
cd02800 (tRNA_bind_EcMetRS_like)

597L

dimer interface
532D, 579F, 596N, 597L, 598K, 601K, 613T, 614T, 615E, 616D, 622L, 624K, 626D, 629V, 633A, 634I, 635V, 636S
CDD
cd02800 (tRNA_bind_EcMetRS_like)

598K

dimer interface
532D, 579F, 596N, 597L, 598K, 601K, 613T, 614T, 615E, 616D, 622L, 624K, 626D, 629V, 633A, 634I, 635V, 636S
CDD
cd02800 (tRNA_bind_EcMetRS_like)

601K

dimer interface
532D, 579F, 596N, 597L, 598K, 601K, 613T, 614T, 615E, 616D, 622L, 624K, 626D, 629V, 633A, 634I, 635V, 636S
CDD
cd02800 (tRNA_bind_EcMetRS_like)

613T

dimer interface
532D, 579F, 596N, 597L, 598K, 601K, 613T, 614T, 615E, 616D, 622L, 624K, 626D, 629V, 633A, 634I, 635V, 636S
CDD
cd02800 (tRNA_bind_EcMetRS_like)

614T

dimer interface
532D, 579F, 596N, 597L, 598K, 601K, 613T, 614T, 615E, 616D, 622L, 624K, 626D, 629V, 633A, 634I, 635V, 636S
CDD
cd02800 (tRNA_bind_EcMetRS_like)

615E

dimer interface
532D, 579F, 596N, 597L, 598K, 601K, 613T, 614T, 615E, 616D, 622L, 624K, 626D, 629V, 633A, 634I, 635V, 636S
CDD
cd02800 (tRNA_bind_EcMetRS_like)

616D

dimer interface
532D, 579F, 596N, 597L, 598K, 601K, 613T, 614T, 615E, 616D, 622L, 624K, 626D, 629V, 633A, 634I, 635V, 636S
CDD
cd02800 (tRNA_bind_EcMetRS_like)

622L

dimer interface
532D, 579F, 596N, 597L, 598K, 601K, 613T, 614T, 615E, 616D, 622L, 624K, 626D, 629V, 633A, 634I, 635V, 636S
CDD
cd02800 (tRNA_bind_EcMetRS_like)

624K

dimer interface
532D, 579F, 596N, 597L, 598K, 601K, 613T, 614T, 615E, 616D, 622L, 624K, 626D, 629V, 633A, 634I, 635V, 636S
CDD
cd02800 (tRNA_bind_EcMetRS_like)

626D

dimer interface
532D, 579F, 596N, 597L, 598K, 601K, 613T, 614T, 615E, 616D, 622L, 624K, 626D, 629V, 633A, 634I, 635V, 636S
CDD
cd02800 (tRNA_bind_EcMetRS_like)

629V

dimer interface
532D, 579F, 596N, 597L, 598K, 601K, 613T, 614T, 615E, 616D, 622L, 624K, 626D, 629V, 633A, 634I, 635V, 636S
CDD
cd02800 (tRNA_bind_EcMetRS_like)

633A

dimer interface
532D, 579F, 596N, 597L, 598K, 601K, 613T, 614T, 615E, 616D, 622L, 624K, 626D, 629V, 633A, 634I, 635V, 636S
CDD
cd02800 (tRNA_bind_EcMetRS_like)

634I

dimer interface
532D, 579F, 596N, 597L, 598K, 601K, 613T, 614T, 615E, 616D, 622L, 624K, 626D, 629V, 633A, 634I, 635V, 636S
CDD
cd02800 (tRNA_bind_EcMetRS_like)

635V

dimer interface
532D, 579F, 596N, 597L, 598K, 601K, 613T, 614T, 615E, 616D, 622L, 624K, 626D, 629V, 633A, 634I, 635V, 636S
CDD
cd02800 (tRNA_bind_EcMetRS_like)

636S

dimer interface
532D, 579F, 596N, 597L, 598K, 601K, 613T, 614T, 615E, 616D, 622L, 624K, 626D, 629V, 633A, 634I, 635V, 636S
CDD
cd02800 (tRNA_bind_EcMetRS_like)
putative tRNA-bind...

557K

putative tRNA-binding site
557K, 569E, 596N, 600T, 607S, 610M
CDD
cd02800 (tRNA_bind_EcMetRS_like)

569E

putative tRNA-binding site
557K, 569E, 596N, 600T, 607S, 610M
CDD
cd02800 (tRNA_bind_EcMetRS_like)

596N

putative tRNA-binding site
557K, 569E, 596N, 600T, 607S, 610M
CDD
cd02800 (tRNA_bind_EcMetRS_like)

600T

putative tRNA-binding site
557K, 569E, 596N, 600T, 607S, 610M
CDD
cd02800 (tRNA_bind_EcMetRS_like)

607S

putative tRNA-binding site
557K, 569E, 596N, 600T, 607S, 610M
CDD
cd02800 (tRNA_bind_EcMetRS_like)

610M

putative tRNA-binding site
557K, 569E, 596N, 600T, 607S, 610M
CDD
cd02800 (tRNA_bind_EcMetRS_like)

GO term prediction

Biological Process

GO:0006418 tRNA aminoacylation for protein translation
GO:0006431 methionyl-tRNA aminoacylation

Molecular Function

GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004825 methionine-tRNA ligase activity
GO:0005524 ATP binding

Cellular Component

GO:0005737 cytoplasm

Filter view on

Entry type

Status

Per-residue features

25586_FusoPortal_Gene_1804

Protein family membership

Homologous superfamilies

Domains and repeats

Detailed signature matches

Residue annotation

GO term prediction

Biological Process

Molecular Function

Cellular Component