49185_FusoPortal_Gene_1588

Protein

49185_FusoPortal_Gene_1588

Length

1,038 amino acids

Protein family membership

Isoleucine-tRNA ligase (IPR002301)
- Isoleucine-tRNA ligase, type 2 (IPR023586)

Homologous superfamilies

Homologous superfamily
38 - 185
Rossmann-like alpha/beta/alpha sandwich fold (IPR014729)
200 - 480
Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain (IPR009008)
643 - 883
Aminoacyl-tRNA synthetase, class Ia, anticodon-binding (IPR009080)

1 100 200 300 400 500 600 700 800 900 1038

Domains and repeats

Domain
20 - 618
Aminoacyl-tRNA synthetase, class Ia (IPR002300)
627 - 825
Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding (IPR013155)
Isoleucyl tRNA synthetase type 2, anticodon-binding domain (IPR033709)

1 100 200 300 400 500 600 700 800 900 1038

Detailed signature matches

IPR014729

Rossmann-like alpha/beta/alpha sandwich fold
1. G3DSA:3.40.50...
  
  38 - 185
  
  GENE3D
  G3DSA:3.40.50.620 (G3DSA:3.40.50.620)
IPR009008

Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain
1. SSF50677 (ValRS/Ile...)
  
  205 - 375
  
  SUPERFAMILY
  SSF50677 (ValRS/IleRS/LeuRS editing domain)
2. G3DSA:3.90.74...
  
  200 - 480
  
  GENE3D
  G3DSA:3.90.740.10 (G3DSA:3.90.740.10)
IPR009080

Aminoacyl-tRNA synthetase, class Ia, anticodon-binding
1. SSF47323 (Anticodon...)
  
  643 - 883
  
  SUPERFAMILY
  SSF47323 (Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases)
IPR002301

Isoleucine-tRNA ligase
1. TIGR00392
  
  5 - 833
  
  TIGRFAMs
  TIGR00392 (TIGR00392)
2. PR00984 (TRNASYNTHILE)
  
  507 - 520
  
  PRINTS
  PR00984 (TRNASYNTHILE)
  
  382 - 397
  
  PRINTS
  PR00984 (TRNASYNTHILE)
  
  41 - 52
  
  PRINTS
  PR00984 (TRNASYNTHILE)
  
  547 - 556
  
  PRINTS
  PR00984 (TRNASYNTHILE)
  
  220 - 243
  
  PRINTS
  PR00984 (TRNASYNTHILE)
IPR023586

Isoleucine-tRNA ligase, type 2
1. MF_02003 (Ile_tRNA_...)
  
  3 - 1,032
  
  HAMAP
  MF_02003 (Ile_tRNA_synth_type2)
IPR002300

Aminoacyl-tRNA synthetase, class Ia
1. PF00133 (tRNA-synt_1)
  
  20 - 618
  
  Pfam
  PF00133 (tRNA-synt_1)
IPR013155

Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding
1. PF08264 (Anticodon_1)
  
  675 - 825
  
  Pfam
  PF08264 (Anticodon_1)
IPR033709

Isoleucyl tRNA synthetase type 2, anticodon-binding domain
1. cd07961 (Anticodon_...)
  
  627 - 810
  
  CDD
  cd07961 (Anticodon_Ia_Ile_ABEc)
IPR001412

Aminoacyl-tRNA synthetase, class I, conserved site
1. PS00178 (AA_TRNA_LI...)
  
  48 - 59
  
  PROSITE patterns
  PS00178 (AA_TRNA_LIGASE_I)

no IPR

Unintegrated signatures

G3DSA:1.10.73...

564 - 862

GENE3D
G3DSA:1.10.730.10 (G3DSA:1.10.730.10)
PTHR42780 (FAMILY N...)

2 - 1,029

PANTHER
PTHR42780 (FAMILY NOT NAMED)
SSF52374 (Nucleotid...)

384 - 631

SUPERFAMILY
SSF52374 (Nucleotidylyl transferase)

7 - 198

SUPERFAMILY
SSF52374 (Nucleotidylyl transferase)
cd00818 (IleRS_core)

39 - 627

CDD
cd00818 (IleRS_core)

Residue annotation

active site cd00818

47P

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

48P

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

49T

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

55H

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

57G

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

58H

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

61T

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

431R

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

515W

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

518S

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

547E

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

548A

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

549V

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

550D

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

551Q

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

553R

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

555W

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

578L

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

579G

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

580H

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

581V

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

582Q

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

586G

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

587Q

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

588K

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

589M

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

590S

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

591K

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)

624P

active site
47P, 48P, 49T, 55H, 57G, 58H, 61T, 431R, 515W, 518S, 547E, 548A, 549V, 550D, 551Q, 553R, 555W, 578L, 579G, 580H, 581V, 582Q, 586G, 587Q, 588K, 589M, 590S, 591K, 624P
CDD
cd00818 (IleRS_core)
HIGH motif cd00818

55H

HIGH motif
55H, 56I, 57G, 58H
CDD
cd00818 (IleRS_core)

56I

HIGH motif
55H, 56I, 57G, 58H
CDD
cd00818 (IleRS_core)

57G

HIGH motif
55H, 56I, 57G, 58H
CDD
cd00818 (IleRS_core)

58H

HIGH motif
55H, 56I, 57G, 58H
CDD
cd00818 (IleRS_core)
KMSKS motif cd00818

588K

KMSKS motif
588K, 589M, 590S, 591K, 592S
CDD
cd00818 (IleRS_core)

589M

KMSKS motif
588K, 589M, 590S, 591K, 592S
CDD
cd00818 (IleRS_core)

590S

KMSKS motif
588K, 589M, 590S, 591K, 592S
CDD
cd00818 (IleRS_core)

591K

KMSKS motif
588K, 589M, 590S, 591K, 592S
CDD
cd00818 (IleRS_core)

592S

KMSKS motif
588K, 589M, 590S, 591K, 592S
CDD
cd00818 (IleRS_core)
tRNA binding surfa...

630K

tRNA binding surface
630K, 634E, 637R, 642T, 645N, 648A, 649F, 699L, 702A, 703R, 706Q, 707D, 710D, 714N, 718R, 721R, 807T, 810R
CDD
cd07961 (Anticodon_Ia_Ile_ABEc)

634E

tRNA binding surface
630K, 634E, 637R, 642T, 645N, 648A, 649F, 699L, 702A, 703R, 706Q, 707D, 710D, 714N, 718R, 721R, 807T, 810R
CDD
cd07961 (Anticodon_Ia_Ile_ABEc)

637R

tRNA binding surface
630K, 634E, 637R, 642T, 645N, 648A, 649F, 699L, 702A, 703R, 706Q, 707D, 710D, 714N, 718R, 721R, 807T, 810R
CDD
cd07961 (Anticodon_Ia_Ile_ABEc)

642T

tRNA binding surface
630K, 634E, 637R, 642T, 645N, 648A, 649F, 699L, 702A, 703R, 706Q, 707D, 710D, 714N, 718R, 721R, 807T, 810R
CDD
cd07961 (Anticodon_Ia_Ile_ABEc)

645N

tRNA binding surface
630K, 634E, 637R, 642T, 645N, 648A, 649F, 699L, 702A, 703R, 706Q, 707D, 710D, 714N, 718R, 721R, 807T, 810R
CDD
cd07961 (Anticodon_Ia_Ile_ABEc)

648A

tRNA binding surface
630K, 634E, 637R, 642T, 645N, 648A, 649F, 699L, 702A, 703R, 706Q, 707D, 710D, 714N, 718R, 721R, 807T, 810R
CDD
cd07961 (Anticodon_Ia_Ile_ABEc)

649F

tRNA binding surface
630K, 634E, 637R, 642T, 645N, 648A, 649F, 699L, 702A, 703R, 706Q, 707D, 710D, 714N, 718R, 721R, 807T, 810R
CDD
cd07961 (Anticodon_Ia_Ile_ABEc)

699L

tRNA binding surface
630K, 634E, 637R, 642T, 645N, 648A, 649F, 699L, 702A, 703R, 706Q, 707D, 710D, 714N, 718R, 721R, 807T, 810R
CDD
cd07961 (Anticodon_Ia_Ile_ABEc)

702A

tRNA binding surface
630K, 634E, 637R, 642T, 645N, 648A, 649F, 699L, 702A, 703R, 706Q, 707D, 710D, 714N, 718R, 721R, 807T, 810R
CDD
cd07961 (Anticodon_Ia_Ile_ABEc)

703R

tRNA binding surface
630K, 634E, 637R, 642T, 645N, 648A, 649F, 699L, 702A, 703R, 706Q, 707D, 710D, 714N, 718R, 721R, 807T, 810R
CDD
cd07961 (Anticodon_Ia_Ile_ABEc)

706Q

tRNA binding surface
630K, 634E, 637R, 642T, 645N, 648A, 649F, 699L, 702A, 703R, 706Q, 707D, 710D, 714N, 718R, 721R, 807T, 810R
CDD
cd07961 (Anticodon_Ia_Ile_ABEc)

707D

tRNA binding surface
630K, 634E, 637R, 642T, 645N, 648A, 649F, 699L, 702A, 703R, 706Q, 707D, 710D, 714N, 718R, 721R, 807T, 810R
CDD
cd07961 (Anticodon_Ia_Ile_ABEc)

710D

tRNA binding surface
630K, 634E, 637R, 642T, 645N, 648A, 649F, 699L, 702A, 703R, 706Q, 707D, 710D, 714N, 718R, 721R, 807T, 810R
CDD
cd07961 (Anticodon_Ia_Ile_ABEc)

714N

tRNA binding surface
630K, 634E, 637R, 642T, 645N, 648A, 649F, 699L, 702A, 703R, 706Q, 707D, 710D, 714N, 718R, 721R, 807T, 810R
CDD
cd07961 (Anticodon_Ia_Ile_ABEc)

718R

tRNA binding surface
630K, 634E, 637R, 642T, 645N, 648A, 649F, 699L, 702A, 703R, 706Q, 707D, 710D, 714N, 718R, 721R, 807T, 810R
CDD
cd07961 (Anticodon_Ia_Ile_ABEc)

721R

tRNA binding surface
630K, 634E, 637R, 642T, 645N, 648A, 649F, 699L, 702A, 703R, 706Q, 707D, 710D, 714N, 718R, 721R, 807T, 810R
CDD
cd07961 (Anticodon_Ia_Ile_ABEc)

807T

tRNA binding surface
630K, 634E, 637R, 642T, 645N, 648A, 649F, 699L, 702A, 703R, 706Q, 707D, 710D, 714N, 718R, 721R, 807T, 810R
CDD
cd07961 (Anticodon_Ia_Ile_ABEc)

810R

tRNA binding surface
630K, 634E, 637R, 642T, 645N, 648A, 649F, 699L, 702A, 703R, 706Q, 707D, 710D, 714N, 718R, 721R, 807T, 810R
CDD
cd07961 (Anticodon_Ia_Ile_ABEc)
anticodon binding ...

645N

anticodon binding site
645N, 648A
CDD
cd07961 (Anticodon_Ia_Ile_ABEc)

648A

anticodon binding site
645N, 648A
CDD
cd07961 (Anticodon_Ia_Ile_ABEc)

GO term prediction

Biological Process

GO:0006418 tRNA aminoacylation for protein translation
GO:0006428 isoleucyl-tRNA aminoacylation

Molecular Function

GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0002161 aminoacyl-tRNA editing activity
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004822 isoleucine-tRNA ligase activity
GO:0005524 ATP binding

Cellular Component

GO:0005737 cytoplasm

Filter view on

Entry type

Status

Per-residue features

49185_FusoPortal_Gene_1588

Protein family membership

Homologous superfamilies

Domains and repeats

Detailed signature matches

Residue annotation

GO term prediction

Biological Process

Molecular Function

Cellular Component