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Protein

49185_FusoPortal_Gene_159

Length

641 amino acids

Protein family membership

Methionyl-tRNA synthetase (IPR014758)
- Methionine-tRNA synthetase, type 2 (IPR023457)

Homologous superfamilies

Homologous superfamily
2 - 345
Rossmann-like alpha/beta/alpha sandwich fold (IPR014729)
354 - 519
Aminoacyl-tRNA synthetase, class Ia, anticodon-binding (IPR009080)
539 - 640
Nucleic acid-binding, OB-fold (IPR012340)

1 100 200 300 400 500 641

Domains and repeats

Domain
4 - 362
Methionyl/Leucyl tRNA synthetase (IPR015413)
Methioninyl-tRNA synthetase core domain (IPR033911)
383 - 481
Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding (IPR013155)
535 - 641
tRNA-binding domain (IPR002547)
Methionyl-tRNA synthetase, beta subunit, C-terminal (IPR004495)

1 100 200 300 400 500 641

Detailed signature matches

IPR014729

Rossmann-like alpha/beta/alpha sandwich fold

G3DSA:3.40.50...

2 - 345

GENE3D
G3DSA:3.40.50.620 (G3DSA:3.40.50.620)

IPR009080

Aminoacyl-tRNA synthetase, class Ia, anticodon-binding

SSF47323 (Anticodon...)

354 - 519

SUPERFAMILY
SSF47323 (Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases)

IPR012340

Nucleic acid-binding, OB-fold

SSF50249 (Nucleic a...)

539 - 640

SUPERFAMILY
SSF50249 (Nucleic acid-binding proteins)

IPR014758

Methionyl-tRNA synthetase

TIGR00398

5 - 484

TIGRFAMs
TIGR00398 (TIGR00398)

IPR023457

Methionine-tRNA synthetase, type 2

MF_01228 (Met_tRNA_...)

1 - 510

HAMAP
MF_01228 (Met_tRNA_synth_type2)

IPR015413

Methionyl/Leucyl tRNA synthetase

PF09334 (tRNA-synt_1g)

5 - 362

Pfam
PF09334 (tRNA-synt_1g)

IPR033911

Methioninyl-tRNA synthetase core domain

PR01041 (TRNASYNTHMET)

257 - 272

PRINTS
PR01041 (TRNASYNTHMET)

349 - 360

PRINTS
PR01041 (TRNASYNTHMET)

7 - 20

PRINTS
PR01041 (TRNASYNTHMET)

39 - 53

PRINTS
PR01041 (TRNASYNTHMET)

223 - 234

PRINTS
PR01041 (TRNASYNTHMET)

87 - 98

PRINTS
PR01041 (TRNASYNTHMET)
cd00814 (MetRS_core)

4 - 337

CDD
cd00814 (MetRS_core)

IPR013155

Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding

PF08264 (Anticodon_1)

383 - 481

Pfam
PF08264 (Anticodon_1)

IPR002547

tRNA-binding domain

PF01588 (tRNA_bind)

545 - 638

Pfam
PF01588 (tRNA_bind)
PS50886 (TRBD)

539 - 641

PROSITE profiles
PS50886 (TRBD)

IPR004495

Methionyl-tRNA synthetase, beta subunit, C-terminal

cd02800 (tRNA_bind_...)

535 - 641

CDD
cd02800 (tRNA_bind_EcMetRS_like)

no IPR

Unintegrated signatures

G3DSA:1.10.73...

346 - 511

GENE3D
G3DSA:1.10.730.10 (G3DSA:1.10.730.10)
G3DSA:2.40.50...

538 - 641

GENE3D
G3DSA:2.40.50.140 (G3DSA:2.40.50.140)
PTHR43326 (FAMILY N...)

2 - 516

PANTHER
PTHR43326 (FAMILY NOT NAMED)
PTHR43326:SF3 (SUBF...)

2 - 516

PANTHER
PTHR43326:SF3 (SUBFAMILY NOT NAMED)
SSF52374 (Nucleotid...)

3 - 352

SUPERFAMILY
SSF52374 (Nucleotidylyl transferase)
cd07957 (Anticodon_...)

346 - 475

CDD
cd07957 (Anticodon_Ia_Met)

Residue annotation

active site cd00814

10P

active site
10P, 11I, 13Y, 50D, 226W, 229A, 230L, 233Y, 263I, 267H
CDD
cd00814 (MetRS_core)

11I

active site
10P, 11I, 13Y, 50D, 226W, 229A, 230L, 233Y, 263I, 267H
CDD
cd00814 (MetRS_core)

13Y

active site
10P, 11I, 13Y, 50D, 226W, 229A, 230L, 233Y, 263I, 267H
CDD
cd00814 (MetRS_core)

50D

active site
10P, 11I, 13Y, 50D, 226W, 229A, 230L, 233Y, 263I, 267H
CDD
cd00814 (MetRS_core)

226W

active site
10P, 11I, 13Y, 50D, 226W, 229A, 230L, 233Y, 263I, 267H
CDD
cd00814 (MetRS_core)

229A

active site
10P, 11I, 13Y, 50D, 226W, 229A, 230L, 233Y, 263I, 267H
CDD
cd00814 (MetRS_core)

230L

active site
10P, 11I, 13Y, 50D, 226W, 229A, 230L, 233Y, 263I, 267H
CDD
cd00814 (MetRS_core)

233Y

active site
10P, 11I, 13Y, 50D, 226W, 229A, 230L, 233Y, 263I, 267H
CDD
cd00814 (MetRS_core)

263I

active site
10P, 11I, 13Y, 50D, 226W, 229A, 230L, 233Y, 263I, 267H
CDD
cd00814 (MetRS_core)

267H

active site
10P, 11I, 13Y, 50D, 226W, 229A, 230L, 233Y, 263I, 267H
CDD
cd00814 (MetRS_core)
HIGH motif cd00814

19H

HIGH motif
19H, 20V, 21G, 22S
CDD
cd00814 (MetRS_core)

20V

HIGH motif
19H, 20V, 21G, 22S
CDD
cd00814 (MetRS_core)

21G

HIGH motif
19H, 20V, 21G, 22S
CDD
cd00814 (MetRS_core)

22S

HIGH motif
19H, 20V, 21G, 22S
CDD
cd00814 (MetRS_core)
KMSKS motif cd00814

298K

KMSKS motif
298K, 299M, 300S, 301K, 302S
CDD
cd00814 (MetRS_core)

299M

KMSKS motif
298K, 299M, 300S, 301K, 302S
CDD
cd00814 (MetRS_core)

300S

KMSKS motif
298K, 299M, 300S, 301K, 302S
CDD
cd00814 (MetRS_core)

301K

KMSKS motif
298K, 299M, 300S, 301K, 302S
CDD
cd00814 (MetRS_core)

302S

KMSKS motif
298K, 299M, 300S, 301K, 302S
CDD
cd00814 (MetRS_core)
tRNA binding surfa...

347N

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

354L

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

355G

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

358L

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

359N

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

362L

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

363G

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

366K

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

367K

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

370N

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

371G

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

421N

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

422K

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

424I

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

425D

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)

430W

tRNA binding surface
347N, 354L, 355G, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 421N, 422K, 424I, 425D, 430W
CDD
cd07957 (Anticodon_Ia_Met)
anticodon binding ...

354L

anticodon binding site
354L, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 424I, 430W
CDD
cd07957 (Anticodon_Ia_Met)

358L

anticodon binding site
354L, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 424I, 430W
CDD
cd07957 (Anticodon_Ia_Met)

359N

anticodon binding site
354L, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 424I, 430W
CDD
cd07957 (Anticodon_Ia_Met)

362L

anticodon binding site
354L, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 424I, 430W
CDD
cd07957 (Anticodon_Ia_Met)

363G

anticodon binding site
354L, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 424I, 430W
CDD
cd07957 (Anticodon_Ia_Met)

366K

anticodon binding site
354L, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 424I, 430W
CDD
cd07957 (Anticodon_Ia_Met)

367K

anticodon binding site
354L, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 424I, 430W
CDD
cd07957 (Anticodon_Ia_Met)

370N

anticodon binding site
354L, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 424I, 430W
CDD
cd07957 (Anticodon_Ia_Met)

371G

anticodon binding site
354L, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 424I, 430W
CDD
cd07957 (Anticodon_Ia_Met)

424I

anticodon binding site
354L, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 424I, 430W
CDD
cd07957 (Anticodon_Ia_Met)

430W

anticodon binding site
354L, 358L, 359N, 362L, 363G, 366K, 367K, 370N, 371G, 424I, 430W
CDD
cd07957 (Anticodon_Ia_Met)
dimer interface cd...

536D

dimer interface
536D, 583S, 600N, 601L, 602K, 605K, 617S, 618T, 619E, 620D, 627L, 629E, 631D, 634V, 638S, 639R, 640A, 641K
CDD
cd02800 (tRNA_bind_EcMetRS_like)

583S

dimer interface
536D, 583S, 600N, 601L, 602K, 605K, 617S, 618T, 619E, 620D, 627L, 629E, 631D, 634V, 638S, 639R, 640A, 641K
CDD
cd02800 (tRNA_bind_EcMetRS_like)

600N

dimer interface
536D, 583S, 600N, 601L, 602K, 605K, 617S, 618T, 619E, 620D, 627L, 629E, 631D, 634V, 638S, 639R, 640A, 641K
CDD
cd02800 (tRNA_bind_EcMetRS_like)

601L

dimer interface
536D, 583S, 600N, 601L, 602K, 605K, 617S, 618T, 619E, 620D, 627L, 629E, 631D, 634V, 638S, 639R, 640A, 641K
CDD
cd02800 (tRNA_bind_EcMetRS_like)

602K

dimer interface
536D, 583S, 600N, 601L, 602K, 605K, 617S, 618T, 619E, 620D, 627L, 629E, 631D, 634V, 638S, 639R, 640A, 641K
CDD
cd02800 (tRNA_bind_EcMetRS_like)

605K

dimer interface
536D, 583S, 600N, 601L, 602K, 605K, 617S, 618T, 619E, 620D, 627L, 629E, 631D, 634V, 638S, 639R, 640A, 641K
CDD
cd02800 (tRNA_bind_EcMetRS_like)

617S

dimer interface
536D, 583S, 600N, 601L, 602K, 605K, 617S, 618T, 619E, 620D, 627L, 629E, 631D, 634V, 638S, 639R, 640A, 641K
CDD
cd02800 (tRNA_bind_EcMetRS_like)

618T

dimer interface
536D, 583S, 600N, 601L, 602K, 605K, 617S, 618T, 619E, 620D, 627L, 629E, 631D, 634V, 638S, 639R, 640A, 641K
CDD
cd02800 (tRNA_bind_EcMetRS_like)

619E

dimer interface
536D, 583S, 600N, 601L, 602K, 605K, 617S, 618T, 619E, 620D, 627L, 629E, 631D, 634V, 638S, 639R, 640A, 641K
CDD
cd02800 (tRNA_bind_EcMetRS_like)

620D

dimer interface
536D, 583S, 600N, 601L, 602K, 605K, 617S, 618T, 619E, 620D, 627L, 629E, 631D, 634V, 638S, 639R, 640A, 641K
CDD
cd02800 (tRNA_bind_EcMetRS_like)

627L

dimer interface
536D, 583S, 600N, 601L, 602K, 605K, 617S, 618T, 619E, 620D, 627L, 629E, 631D, 634V, 638S, 639R, 640A, 641K
CDD
cd02800 (tRNA_bind_EcMetRS_like)

629E

dimer interface
536D, 583S, 600N, 601L, 602K, 605K, 617S, 618T, 619E, 620D, 627L, 629E, 631D, 634V, 638S, 639R, 640A, 641K
CDD
cd02800 (tRNA_bind_EcMetRS_like)

631D

dimer interface
536D, 583S, 600N, 601L, 602K, 605K, 617S, 618T, 619E, 620D, 627L, 629E, 631D, 634V, 638S, 639R, 640A, 641K
CDD
cd02800 (tRNA_bind_EcMetRS_like)

634V

dimer interface
536D, 583S, 600N, 601L, 602K, 605K, 617S, 618T, 619E, 620D, 627L, 629E, 631D, 634V, 638S, 639R, 640A, 641K
CDD
cd02800 (tRNA_bind_EcMetRS_like)

638S

dimer interface
536D, 583S, 600N, 601L, 602K, 605K, 617S, 618T, 619E, 620D, 627L, 629E, 631D, 634V, 638S, 639R, 640A, 641K
CDD
cd02800 (tRNA_bind_EcMetRS_like)

639R

dimer interface
536D, 583S, 600N, 601L, 602K, 605K, 617S, 618T, 619E, 620D, 627L, 629E, 631D, 634V, 638S, 639R, 640A, 641K
CDD
cd02800 (tRNA_bind_EcMetRS_like)

640A

dimer interface
536D, 583S, 600N, 601L, 602K, 605K, 617S, 618T, 619E, 620D, 627L, 629E, 631D, 634V, 638S, 639R, 640A, 641K
CDD
cd02800 (tRNA_bind_EcMetRS_like)

641K

dimer interface
536D, 583S, 600N, 601L, 602K, 605K, 617S, 618T, 619E, 620D, 627L, 629E, 631D, 634V, 638S, 639R, 640A, 641K
CDD
cd02800 (tRNA_bind_EcMetRS_like)
putative tRNA-bind...

561K

putative tRNA-binding site
561K, 573A, 600N, 604V, 611S, 614M
CDD
cd02800 (tRNA_bind_EcMetRS_like)

573A

putative tRNA-binding site
561K, 573A, 600N, 604V, 611S, 614M
CDD
cd02800 (tRNA_bind_EcMetRS_like)

600N

putative tRNA-binding site
561K, 573A, 600N, 604V, 611S, 614M
CDD
cd02800 (tRNA_bind_EcMetRS_like)

604V

putative tRNA-binding site
561K, 573A, 600N, 604V, 611S, 614M
CDD
cd02800 (tRNA_bind_EcMetRS_like)

611S

putative tRNA-binding site
561K, 573A, 600N, 604V, 611S, 614M
CDD
cd02800 (tRNA_bind_EcMetRS_like)

614M

putative tRNA-binding site
561K, 573A, 600N, 604V, 611S, 614M
CDD
cd02800 (tRNA_bind_EcMetRS_like)

GO term prediction

Biological Process

GO:0006418 tRNA aminoacylation for protein translation
GO:0006431 methionyl-tRNA aminoacylation

Molecular Function

GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004825 methionine-tRNA ligase activity
GO:0005524 ATP binding

Cellular Component

GO:0005737 cytoplasm