9817_FusoPortal_Gene_2197

Protein

9817_FusoPortal_Gene_2197

Length

571 amino acids

Protein family membership

Acetolactate synthase, large subunit, biosynthetic (IPR012846)

Homologous superfamilies

Homologous superfamily
2 - 177
Thiamin diphosphate-binding fold (IPR029061)
195 - 361
DHS-like NAD/FAD-binding domain superfamily (IPR029035)
369 - 564
Thiamin diphosphate-binding fold (IPR029061)

1 100 200 300 400 500 571

Domains and repeats

Domain
7 - 174
Thiamine pyrophosphate enzyme, N-terminal TPP-binding domain (IPR012001)
203 - 335
Thiamine pyrophosphate enzyme, central domain (IPR012000)
393 - 540
Thiamine pyrophosphate enzyme, C-terminal TPP-binding (IPR011766)

1 100 200 300 400 500 571

Detailed signature matches

IPR029061

Thiamin diphosphate-binding fold
1. SSF52518 (Thiamin d...)
  
  2 - 177
  
  SUPERFAMILY
  SSF52518 (Thiamin diphosphate-binding fold (THDP-binding))
  
  369 - 564
  
  SUPERFAMILY
  SSF52518 (Thiamin diphosphate-binding fold (THDP-binding))
IPR029035

DHS-like NAD/FAD-binding domain superfamily
1. SSF52467 (DHS-like ...)
  
  196 - 361
  
  SUPERFAMILY
  SSF52467 (DHS-like NAD/FAD-binding domain)
2. G3DSA:3.40.50...
  
  195 - 351
  
  GENE3D
  G3DSA:3.40.50.1220 (G3DSA:3.40.50.1220)
IPR012846

Acetolactate synthase, large subunit, biosynthetic
1. TIGR00118
  
  7 - 563
  
  TIGRFAMs
  TIGR00118 (TIGR00118)
IPR012001

Thiamine pyrophosphate enzyme, N-terminal TPP-binding domain
1. PF02776 (TPP_enzyme_N)
  
  7 - 174
  
  Pfam
  PF02776 (TPP_enzyme_N)
IPR012000

Thiamine pyrophosphate enzyme, central domain
1. PF00205 (TPP_enzyme_M)
  
  203 - 335
  
  Pfam
  PF00205 (TPP_enzyme_M)
IPR011766

Thiamine pyrophosphate enzyme, C-terminal TPP-binding
1. PF02775 (TPP_enzyme_C)
  
  393 - 540
  
  Pfam
  PF02775 (TPP_enzyme_C)
IPR000399

TPP-binding enzyme, conserved site
1. PS00187 (TPP_ENZYMES)
  
  430 - 449
  
  PROSITE patterns
  PS00187 (TPP_ENZYMES)

no IPR

Unintegrated signatures

G3DSA:3.40.50...

1 - 194

GENE3D
G3DSA:3.40.50.970 (G3DSA:3.40.50.970)

352 - 561

GENE3D
G3DSA:3.40.50.970 (G3DSA:3.40.50.970)
PTHR18968 (THIAMINE...)

4 - 563

PANTHER
PTHR18968 (THIAMINE PYROPHOSPHATE ENZYMES)
PTHR18968:SF116 (AC...)

4 - 563

PANTHER
PTHR18968:SF116 (ACETOLACTATE SYNTHASE ISOZYME 3 LARGE SUBUNIT)
cd02015 (TPP_AHAS)

374 - 555

CDD
cd02015 (TPP_AHAS)
cd02440 (AdoMet_MTases)

301 - 390

CDD
cd02440 (AdoMet_MTases)
cd07035 (TPP_PYR_PO...)

11 - 165

CDD
cd07035 (TPP_PYR_POX_like)

Residue annotation

PYR/PP interface c...

23D

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

28P

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

29G

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

33I

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

37D

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

46K

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

47H

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

48Y

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

49L

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

50S

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

51R

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

52H

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

55G

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

58H

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

59E

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

62G

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

63Y

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

65R

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

66A

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

67S

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

79P

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

86T

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

90T

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

93M

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)

94D

PYR/PP interface
23D, 28P, 29G, 33I, 37D, 46K, 47H, 48Y, 49L, 50S, 51R, 52H, 55G, 58H, 59E, 62G, 63Y, 65R, 66A, 67S, 79P, 86T, 90T, 93M, 94D
CDD
cd07035 (TPP_PYR_POX_like)
TPP binding site c...

28P

TPP binding site
28P, 53E, 79P, 83N
CDD
cd07035 (TPP_PYR_POX_like)

53E

TPP binding site
28P, 53E, 79P, 83N
CDD
cd07035 (TPP_PYR_POX_like)

79P

TPP binding site
28P, 53E, 79P, 83N
CDD
cd07035 (TPP_PYR_POX_like)

83N

TPP binding site
28P, 53E, 79P, 83N
CDD
cd07035 (TPP_PYR_POX_like)
dimer interface cd...

28P

dimer interface
28P, 29G, 30G, 33I, 37D, 47H, 49L, 50S, 51R, 52H, 54Q, 79P, 82T, 83N, 85V, 86T, 89M, 123I, 126P
CDD
cd07035 (TPP_PYR_POX_like)

29G

dimer interface
28P, 29G, 30G, 33I, 37D, 47H, 49L, 50S, 51R, 52H, 54Q, 79P, 82T, 83N, 85V, 86T, 89M, 123I, 126P
CDD
cd07035 (TPP_PYR_POX_like)

30G

dimer interface
28P, 29G, 30G, 33I, 37D, 47H, 49L, 50S, 51R, 52H, 54Q, 79P, 82T, 83N, 85V, 86T, 89M, 123I, 126P
CDD
cd07035 (TPP_PYR_POX_like)

33I

dimer interface
28P, 29G, 30G, 33I, 37D, 47H, 49L, 50S, 51R, 52H, 54Q, 79P, 82T, 83N, 85V, 86T, 89M, 123I, 126P
CDD
cd07035 (TPP_PYR_POX_like)

37D

dimer interface
28P, 29G, 30G, 33I, 37D, 47H, 49L, 50S, 51R, 52H, 54Q, 79P, 82T, 83N, 85V, 86T, 89M, 123I, 126P
CDD
cd07035 (TPP_PYR_POX_like)

47H

dimer interface
28P, 29G, 30G, 33I, 37D, 47H, 49L, 50S, 51R, 52H, 54Q, 79P, 82T, 83N, 85V, 86T, 89M, 123I, 126P
CDD
cd07035 (TPP_PYR_POX_like)

49L

dimer interface
28P, 29G, 30G, 33I, 37D, 47H, 49L, 50S, 51R, 52H, 54Q, 79P, 82T, 83N, 85V, 86T, 89M, 123I, 126P
CDD
cd07035 (TPP_PYR_POX_like)

50S

dimer interface
28P, 29G, 30G, 33I, 37D, 47H, 49L, 50S, 51R, 52H, 54Q, 79P, 82T, 83N, 85V, 86T, 89M, 123I, 126P
CDD
cd07035 (TPP_PYR_POX_like)

51R

dimer interface
28P, 29G, 30G, 33I, 37D, 47H, 49L, 50S, 51R, 52H, 54Q, 79P, 82T, 83N, 85V, 86T, 89M, 123I, 126P
CDD
cd07035 (TPP_PYR_POX_like)

52H

dimer interface
28P, 29G, 30G, 33I, 37D, 47H, 49L, 50S, 51R, 52H, 54Q, 79P, 82T, 83N, 85V, 86T, 89M, 123I, 126P
CDD
cd07035 (TPP_PYR_POX_like)

54Q

dimer interface
28P, 29G, 30G, 33I, 37D, 47H, 49L, 50S, 51R, 52H, 54Q, 79P, 82T, 83N, 85V, 86T, 89M, 123I, 126P
CDD
cd07035 (TPP_PYR_POX_like)

79P

dimer interface
28P, 29G, 30G, 33I, 37D, 47H, 49L, 50S, 51R, 52H, 54Q, 79P, 82T, 83N, 85V, 86T, 89M, 123I, 126P
CDD
cd07035 (TPP_PYR_POX_like)

82T

dimer interface
28P, 29G, 30G, 33I, 37D, 47H, 49L, 50S, 51R, 52H, 54Q, 79P, 82T, 83N, 85V, 86T, 89M, 123I, 126P
CDD
cd07035 (TPP_PYR_POX_like)

83N

dimer interface
28P, 29G, 30G, 33I, 37D, 47H, 49L, 50S, 51R, 52H, 54Q, 79P, 82T, 83N, 85V, 86T, 89M, 123I, 126P
CDD
cd07035 (TPP_PYR_POX_like)

85V

dimer interface
28P, 29G, 30G, 33I, 37D, 47H, 49L, 50S, 51R, 52H, 54Q, 79P, 82T, 83N, 85V, 86T, 89M, 123I, 126P
CDD
cd07035 (TPP_PYR_POX_like)

86T

dimer interface
28P, 29G, 30G, 33I, 37D, 47H, 49L, 50S, 51R, 52H, 54Q, 79P, 82T, 83N, 85V, 86T, 89M, 123I, 126P
CDD
cd07035 (TPP_PYR_POX_like)

89M

dimer interface
28P, 29G, 30G, 33I, 37D, 47H, 49L, 50S, 51R, 52H, 54Q, 79P, 82T, 83N, 85V, 86T, 89M, 123I, 126P
CDD
cd07035 (TPP_PYR_POX_like)

123I

dimer interface
28P, 29G, 30G, 33I, 37D, 47H, 49L, 50S, 51R, 52H, 54Q, 79P, 82T, 83N, 85V, 86T, 89M, 123I, 126P
CDD
cd07035 (TPP_PYR_POX_like)

126P

dimer interface
28P, 29G, 30G, 33I, 37D, 47H, 49L, 50S, 51R, 52H, 54Q, 79P, 82T, 83N, 85V, 86T, 89M, 123I, 126P
CDD
cd07035 (TPP_PYR_POX_like)
S-adenosylmethioni...

309D

S-adenosylmethionine binding site
309D, 310I, 344E, 345D, 346I
CDD
cd02440 (AdoMet_MTases)

310I

S-adenosylmethionine binding site
309D, 310I, 344E, 345D, 346I
CDD
cd02440 (AdoMet_MTases)

344E

S-adenosylmethionine binding site
309D, 310I, 344E, 345D, 346I
CDD
cd02440 (AdoMet_MTases)

345D

S-adenosylmethionine binding site
309D, 310I, 344E, 345D, 346I
CDD
cd02440 (AdoMet_MTases)

346I

S-adenosylmethionine binding site
309D, 310I, 344E, 345D, 346I
CDD
cd02440 (AdoMet_MTases)
TPP-binding site c...

394V

TPP-binding site
394V, 395G, 396Q, 397H, 420G, 422M, 447D, 448G, 449G, 452M, 474N
CDD
cd02015 (TPP_AHAS)

395G

TPP-binding site
394V, 395G, 396Q, 397H, 420G, 422M, 447D, 448G, 449G, 452M, 474N
CDD
cd02015 (TPP_AHAS)

396Q

TPP-binding site
394V, 395G, 396Q, 397H, 420G, 422M, 447D, 448G, 449G, 452M, 474N
CDD
cd02015 (TPP_AHAS)

397H

TPP-binding site
394V, 395G, 396Q, 397H, 420G, 422M, 447D, 448G, 449G, 452M, 474N
CDD
cd02015 (TPP_AHAS)

420G

TPP-binding site
394V, 395G, 396Q, 397H, 420G, 422M, 447D, 448G, 449G, 452M, 474N
CDD
cd02015 (TPP_AHAS)

422M

TPP-binding site
394V, 395G, 396Q, 397H, 420G, 422M, 447D, 448G, 449G, 452M, 474N
CDD
cd02015 (TPP_AHAS)

447D

TPP-binding site
394V, 395G, 396Q, 397H, 420G, 422M, 447D, 448G, 449G, 452M, 474N
CDD
cd02015 (TPP_AHAS)

448G

TPP-binding site
394V, 395G, 396Q, 397H, 420G, 422M, 447D, 448G, 449G, 452M, 474N
CDD
cd02015 (TPP_AHAS)

449G

TPP-binding site
394V, 395G, 396Q, 397H, 420G, 422M, 447D, 448G, 449G, 452M, 474N
CDD
cd02015 (TPP_AHAS)

452M

TPP-binding site
394V, 395G, 396Q, 397H, 420G, 422M, 447D, 448G, 449G, 452M, 474N
CDD
cd02015 (TPP_AHAS)

474N

TPP-binding site
394V, 395G, 396Q, 397H, 420G, 422M, 447D, 448G, 449G, 452M, 474N
CDD
cd02015 (TPP_AHAS)
dimer interface cd...

417G

dimer interface
417G, 420G, 422M, 451Q, 452M, 454I, 455Q, 462A, 501P, 502D, 506V, 509A
CDD
cd02015 (TPP_AHAS)

420G

dimer interface
417G, 420G, 422M, 451Q, 452M, 454I, 455Q, 462A, 501P, 502D, 506V, 509A
CDD
cd02015 (TPP_AHAS)

422M

dimer interface
417G, 420G, 422M, 451Q, 452M, 454I, 455Q, 462A, 501P, 502D, 506V, 509A
CDD
cd02015 (TPP_AHAS)

451Q

dimer interface
417G, 420G, 422M, 451Q, 452M, 454I, 455Q, 462A, 501P, 502D, 506V, 509A
CDD
cd02015 (TPP_AHAS)

452M

dimer interface
417G, 420G, 422M, 451Q, 452M, 454I, 455Q, 462A, 501P, 502D, 506V, 509A
CDD
cd02015 (TPP_AHAS)

454I

dimer interface
417G, 420G, 422M, 451Q, 452M, 454I, 455Q, 462A, 501P, 502D, 506V, 509A
CDD
cd02015 (TPP_AHAS)

455Q

dimer interface
417G, 420G, 422M, 451Q, 452M, 454I, 455Q, 462A, 501P, 502D, 506V, 509A
CDD
cd02015 (TPP_AHAS)

462A

dimer interface
417G, 420G, 422M, 451Q, 452M, 454I, 455Q, 462A, 501P, 502D, 506V, 509A
CDD
cd02015 (TPP_AHAS)

501P

dimer interface
417G, 420G, 422M, 451Q, 452M, 454I, 455Q, 462A, 501P, 502D, 506V, 509A
CDD
cd02015 (TPP_AHAS)

502D

dimer interface
417G, 420G, 422M, 451Q, 452M, 454I, 455Q, 462A, 501P, 502D, 506V, 509A
CDD
cd02015 (TPP_AHAS)

506V

dimer interface
417G, 420G, 422M, 451Q, 452M, 454I, 455Q, 462A, 501P, 502D, 506V, 509A
CDD
cd02015 (TPP_AHAS)

509A

dimer interface
417G, 420G, 422M, 451Q, 452M, 454I, 455Q, 462A, 501P, 502D, 506V, 509A
CDD
cd02015 (TPP_AHAS)

GO term prediction

Biological Process

GO:0009082 branched-chain amino acid biosynthetic process

Molecular Function

GO:0000287 magnesium ion binding
GO:0003824 catalytic activity
GO:0003984 acetolactate synthase activity
GO:0030976 thiamine pyrophosphate binding
GO:0050660 flavin adenine dinucleotide binding

Cellular Component

None predicted.

Filter view on

Entry type

Status

Per-residue features

9817_FusoPortal_Gene_2197

Protein family membership

Homologous superfamilies

Domains and repeats

Detailed signature matches

Residue annotation

GO term prediction

Biological Process

Molecular Function

Cellular Component